Growing melon (Cucumis melo L.) still faces many difficulties due to vine death disease caused by Fusarium sp. In the present study, melon plants with wilt disease symptoms were collected from Phong Dien (PD) and Cai Rang (CR) districts, Can Tho city (Vietnam). Three strains of Fusarium sp. (CRI, CR8.1 and PD) were isolated based on morphological characteristics such as the color of the fungus, spore sepals, branching patterns from the fungal hyphae, crescent-shaped megaspores measuring 24.7 - 27.34 x 3.66 - 3.68 µm; thin-walled spherical mantle spores. Among them, Fusarium isolate CRI had the highest virulence with a pathogenicity index of 96.8 % and a disease incidence of 100 % after 15 days of inoculation. The research results showed that the two rootstocks, pumpkin and luffa, had the lowest mortality rate (0.0 %) at all survey times and were highly resistant to string rot disease on melon caused by Fusarium sp.