Molecular characterization of Begomovirus and Beta satellite virus associated with leaf curl disease of Chilli (Capsicum annum L.) in southern parts of Tamil Nadu
DOI:
https://doi.org/10.14719/pst.4712Keywords:
Coat protein, Gemini virus, Chilli leaf curl virus, DNA-AAbstract
Chilli is one of the widely cultivated commercial vegetable crops in India. Chilli leaf curl is the most devasting disease and causes a yield loss of 100 per cent in Chilli. Fourteen begomovirus species cause chilli leaf curl disease (ChiLCD). An extensive survey was carried out in 2023 to determine the prevalence of the leaf curl disease of Chilli caused by begomovirus in major chilli-growing areas of the Southern Districts of Tamil Nadu. During the survey, 21 isolates of begomovirus infecting Chilli were collected. Among the 21 isolates, the isolate collected from Kalakudi village (BV-KI) exhibited severe symptoms.DNA extracted from 21 isolates of begomovirus were subjected to PCR by using Rojas universal primers, Chilli leaf curl virus (ChiLCV) Coat protein primers and beta satellite virus primers and obtained 1380 bp, 1100 bp and 1400 bp amplicons of top and coat protein region of DNA-A of begomovirus, Chilli leaf curl virus coat protein region and beta satellite virus associated with ChiLCV respectively. The 1380 bp, 1100 bp and 1400 bp amplicons obtained from the BV-KI isolate were sequenced and submitted to the NCBI. The sequences were blast analyzed, and it showed that the nucleotide sequence of the top and coat protein region of DNA-A of begomovirus and CP gene of ChiLCV BV-KI isolate was found to have 90.61 and 99.78% similarity with Bhavanisagar (NC 055130.1) isolate respectively. The nucleotide sequence of the beta satellite virus of BV-KI isolate was found to have 99.93% similarity with the Chilli leaf curl beta satellite virus Meerut (MH355642.1) isolate.
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References
Subbiah A, Jeyakumar S. Production and marketing of chillies. Facts You. 2009; 29 (6):1-19.
Spice Board India. Ministry of Commerce and Industry 2022-2023,First Advance Estimate of spices, Government of India. 2023.
Varma A, Malathi V. Emerging geminivirus problems: a serious threat to crop production. Annals of Applied Biology. 2003; 142 (2):145-64. https://doi.org/10.1111/j.1744-7348.2003.tb00240.x
Kheyr-Pour A, Bendahmane M, Matzeit V, Accotto GP, Crespi S, Gronenborn B. Tomato yellow leaf curl virus from sardinia is a whitefly-transmitted monoparatite geminivirus. Nucleic acids research.1991;19(24): 6763-769. https://doi.org/10.1093/nar/19.24.6763
Chakraborty S, Pandey P, Banerjee M, Kalloo G, Fauquet C. Tomato leaf curl Gujarat virus, a new begomovirus species causing a severe leaf curl disease of tomato in Varanasi, India. Phytopathology. 2003; 93(12): 1485-495. https://doi.org/10.1094/PHYTO.2003.93.12.1485
Briddon R, Stanley J. Subviral agents associated with plant single-stranded DNA viruses. Virology. 2006; 344(1):198-10. https://doi.org/10.1016/j.virol.2005.09.042
Fondong VN. Gemini virus protein structure and function. Molecular plant pathology. 2013; 14(6): 635-49. https://doi.org/10.1111/mpp.12032
Venkataravanappa V, Swarnalatha P, Reddy CL, Chauhan N, Reddy MK. Association of recombinant Chilli leaf curl virus with enation leaf curl disease of tomato: a new host for chilli begomovirus in India. Phytoparasitica. 2016; 44: 213-23. https://doi.org/10.1007/s12600-016-0510-9
Senanayake D, Mandal B, Lodha S, Varma A. First report of Chilli leaf curl virus affecting Chilli in India. Plant pathology.2007;56(2). https://doi.org/10.1111/j.1365-3059.2007.01513.x
Senanayake D, Varma A, Mandal B. Virus–vector relationships, host range, detection and sequence comparison of Chilli leaf curl virus associated with an epidemic of leaf curl disease of Chilli in Jodhpur, India. Journal of Phytopathology. 2012;160(3):146-55. https://doi.org/10.1111/j.1439-0434.2011.01876.x
Malathi V, Renukadevi P, Chakraborty S, Biswas K, et al. Begomo viruses and their satellites occurring in India: distribution, diversity and pathogenesis. A century of plant virology in India. 2017; 75-177. https://doi.org/10.1007/978-981-10-5672-7_5
Shingote PR, Wasule DL, Parma VS, Holkar SK, et al. An overview of chili leaf curl disease: Molecular mechanisms, impact, challenges, and disease management strategies in Indian subcontinent. Frontiers in Microbiology. 2022;13. https://doi.org/10.3389/fmicb.2022.899512
Wahyono A, Murti R.H, Hartono S, Nuringtyas T.R, etal. Current status and complexity of three Begomovirus species in pepper plants in lowlands and highlands in Java Island, Indonesia. Viruses. 2023; 15 (6). https://doi.org/10.3390/v15061278
Lavanya R, Arun V. Standardization of DNA extraction protocol and screening of begomovirus in chilli and tomato plants. Journal of Plant Diseases and Protection. 2019; 126 (4):343-49. https://doi.org/10.1007/s41348-019-00228-6
Rojas MR. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant disease.1993;77(4): 340. https://doi.org/10.1094/PD-77-0340
Kumar, R.V., A. Singh, and S. Chakraborty. A new monopartite begomovirus species, Chilli leaf curl Vellanad virus and associated betasatellites infecting Chilli in the Vellanad region of Kerala, India. New Dis Rep. 2012; 25 (20):2044-0588. https://doi.org/10.5197/j.2044-0588.2012.025.020
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Copyright (c) 2024 N. Rajinimala, J. Muthamil Kumaran, K. Elanchezhyan, E. Santhoshinii, M. Theradimani, M.I. Manivannan, J. Sheela
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