Rice straw biostimulants: Phytochemicals and effects on maize growth and tomato yield
DOI:
https://doi.org/10.14719/pst.5111Keywords:
rice straw, biostimulants, cow urine, organic farming, phytochemical profiling, maize, tomatoAbstract
Biostimulants offer sustainable alternatives to enhance plant growth, resilience and crop yield, especially in organic farming. This study aimed to develop a nutrient-enriched biostimulant from rice straw, a widely available agricultural by-product, often burned, leading to environmental pollution. Rice straw was collected, processed and extracted using cow urine and deionized water (1:50). The extracts underwent phytochemical analysis via GC-MS and LC-MS, identifying bioactive compounds such as fatty acids, sterols, phenols and flavonoids. Elemental analysis confirmed the presence of key nutrients and trace elements, including potassium and magnesium, essential for plant growth. In-vitro assays on maize (CO6 hybrid) evaluated the biostimulant’s efficacy, showing a 12 % increase in germination and a 25 % improvement in seedling vigor at optimal cow urine extract concentrations (CE) (25 %). Root length and shoot biomass also exhibited significant improvements. Field experiments on tomato cultivar (Madhan hybrid) compared the effects of the rice straw-based biostimulant with panchagavya, a traditional organic preparation, alongside controls including cow urine and water. The findings revealed that the rice straw-derived biostimulant markedly improved fruit yield by 22 % compared to the control. This enhancement exceeded that of panchagavya, which achieved a 17 % increase in fruit yield. These results underscore the superior efficacy of the rice straw-based treatment, especially those extracted with cow urine, in enhancing crop productivity, reducing reliance on synthetic agrochemicals and promoting environmentally sustainable agricultural practices.
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Yakhin OI, Aleksandr A, Lubyanov IA, Yakhin, Brown PH. Biostimulants in plant science: a global perspective. Front Plant Sci. 2017;7:238366. https://doi.org/10.3389/fpls.2016.02049
Rouphael Y, Colla G. Biostimulants in agriculture. Front Plant Sci. 2020;11:511937.https://doi.org/10.3389/fpls.2020.00040
Ma X, Hu J, Wang X, Choi S, Zhang T, et al. An integrated strategy for the utilization of rice straw: Production of plant growth promoter followed by ethanol fermentation. Process Saf Environ Prot. 2019;129:1-7.https://doi.org/10.1016/j.psep.2019.06.004
ChenX, Xue Y, Hu J, Tsang YF, Gao MT. Release of polyphenols is the major factor influencing the bioconversion of rice straw to lactic acid. Appl Biochem Biotechnol. 2017;183:685-98.https://doi.org/10.1007/s12010-017-2457-9
AdhikarySK, Ashish DK, Rudzionis Z. A review on sustainable use of agricultural straw and husk biomass ashes: Transitioning towards low carbon economy. Sci Total Environ. 2022;838. https://doi.org/10.1016/j.scitotenv.2022.156407
KingraPK, Bora S, Singh Y. Crop residue burning and its implications over North-West India. J Agric Phys. 2021;21(2):316-31.
Urban Cordeiro E, Arenas-Calle L, Woolf D, Sherpa S, et al. The fate of rice crop residues and context-dependent greenhouse gas emissions: Model-based insights from Eastern India. J Clean Prod. 2024;5435. https://doi.org/10.1016/j.jclepro.2023.140240
Jain N, Bhatia A, Pathak H. Emission of air pollutants from crop residue burning in India. Aerosol Air Qual Res. 2014;14:422-30.https://doi.org/10.4209/aaqr.2013.01.0031
Toan NS, Phuong NTD, Thuy PT, Dong PD, et al. Effects of burning rice straw residue on-field on soil organic carbon pools: Environment-friendly approach from a conventional rice paddy in central Vietnam. Chemosphere. 2022;294. https://doi.org/10.1016/j.chemosphere.2022.133596
Tanase C, Co?arca S, Muntean DL. A critical review of phenolic compounds extracted from the bark of woody vascular plants and their potential biological activity. Molecules. 2019;6:1182.https://doi.org/10.3390/molecules24061182
Peanparkdee M, Iwamoto S. Bioactive compounds from by-products of rice cultivation and rice processing: Extraction and application in the food and pharmaceutical industries. Trends Food Sci Technol. 2019;86:109-17.https://doi.org/10.1016/j.tifs.2019.02.041
Kumar A, Nayak AK, Sharma S, Senapati A, Mitra D, Mohanty B, et al. Rice straw recycling: A sustainable approach for ensuring environmental quality and economic security. Pedosphere. 2023;33(1):34-48.https://doi.org/10.1016/j.pedsph.2022.06.036
Aslam Z, Zaman Q, Ihsan MZ, Syed S, Fujii Y, et al. Efficacy of rice straw extracts in controlling weeds and enhancing the productivity of wheat (Triticum aestivum L.). Pak J Weed Sci Res. 2016;22(2):197-210.
Barus Y. Application of rice straw compost with different bioactivators on the growth and yield of rice plant. J Trop Soils. 2019;17:25-29. http://dx.doi.org/10.5400/jts.2012.v17i1.25-29
Sadhukhan R, Bohra JS, Sen S. Growth and yield response of rice (Oryza sativa) to graded doses of nutrients and foliar spray of cow urine. Indian J Agron. 2019;64:58-61. https://doi.org/10.59797/ija.v64i1.5233
Vaghela P, Das AK, Trivedi K, Anand KV, et al. Characterization and metabolomics profiling of Kappaphycus alvarezii seaweed extract. Algal Res. 2022;66:102774. https://doi.org/10.1016/j.algal.2022.102774
Jaiswal PC. Soil, plant and water analysis. New Delhi: Kalyani Publishers. 2003. ISBN. 81-272-0690-3.
AOSA - Association of Official Seed Analysts. Seed Vigor Testing Handbook. 1983;93.
Orchard TJ. Estimating the parameters of plant seedling emergence. Seed Sci Technol. 1977;5(1):61-69.
Ellis RH, Roberts EH. The quantification of ageing and survival in orthodox seeds. Seed Sci Technol (Netherlands). 1981;9(2):373-409.
Sheoran OP, Tonk DS, Kaushik LS, Hasija RC, Pannu RS. Statistical software package for agricultural research workers. Recent Advances in Information Theory, Statistics and Computer Applications by Hooda D.S., Hasija R.C. Hisar: CCS HAU. 1998;139-43.
Deeksha TR, Sharma M, Parmar M. The Effect of different pre-sowing treatments of cow urine, soaking duration, PGPR applications and their combinations on seed germination and seedling growth parameters of custard apple (Annona squamosa L.). Asian J Agric Hort Res. 2023;10:527-37.https://doi.org/10.9734/ajahr/2023/v10i4292
Nautiyal V, Dubey RC. FT-IR and GC-MS analyses of potential bioactive compounds of cow urine and its antibacterial activity. Saudi J Biol. Sci. 2021;28(4):2432-37. https://doi.org/10.1016/j.sjbs.2021.01.041
Xiao B, Sun XF, Sun RC. Extraction and characterization of lipophilic extractives from rice straw. J Wood Chem Technol. 2001;21:397-411. https://doi.org/10.1081/WCT-100108334
Hakim Z, Hussain A, Shabir M, Aslam A, Hassan M, Waqas SUD, et al. The effect of foliar application of benzoic acid on growth and production of wheat. Haya Saudi J Life Sci. 2023;8(11):260-65. https://doi.org/10.36348/sjls.2023.v08i11.005
Stringlis IA, De Jonge R, Pieterse CMJ. The age of coumarins in plant–microbe interactions. Plant Cell Physiol. 2019;60:1405-19.https://doi.org/10.1093/pcp/pcz076
Phitsuwan P, Permsriburasuk C, Baramee S, Teeravivattanakit T, Ratanakhanokchai K. Structural analysis of alkaline pretreated rice straw for ethanol production. Int J Polym Sci. 2017;(1):4876969.https://doi.org/10.1155/2017/4876969
Khalid M, Bilal M, Dan-feng H. Role of flavonoids in plant interactions with the environment and against human pathogens-A review. J Integr Agric. 2019;18(1):211-30.https://doi.org/10.1016/S2095-3119(19)62555-4
Zhang P, Zhang H, Wu G, Chen X, Gruda N, et al. Dose-dependent application of straw-derived fulvic acid on yield and quality of tomato plants grown in a greenhouse. Front Plant Sci. 2021;12:736613.https://doi.org/10.3389/fpls.2021.736613
Lingzhi S, Yuming F, Wenting F, Min Y, et al. Effects of aqueous extract of soil-like substrate made from three different materials on seed germination and seedling growth of rice. Acta Astronaut. 2014;96:83-88. https://doi.org/10.1016/j.actaastro.2013.10.007
Sharma P, Yadav RK, Jain MC, Bhateshwar MC. Growing media and cow urine influence the seed germination and seedling growth of Papaya (Carica papaya L.). J Crop Weed. 2021;17(3):253-59. https://doi.org/10.22271/09746315.2021.v17.i3.1520
Kinnari NB, Dilipkumar DP. Effects of cow urine and medicinal plants as seed treatment on germination rate and growth of Cluster bean (Cyamopsis tetragonoloba) and Fenugreek (Trigonella foenum-graecum). Int J Sci Res Sci Technol. 2023;10(1):381-405.https://doi.org/10.32628/IJSRST2310147
Meselhy KM, Shams MM, Sherif NH, El-Sonbaty SM. Phenolic profile and in vivo cytotoxic activity of rice straw extract. Pharmacogn J. 2019;11(5):849-57. http://dx.doi.org/10.5530/pj.2019.11.137
Jumrus S, Yamuangmorn S, Veeradittakit J, Kamthai S, et al. Variation of anthocyanin, phenol and antioxidant capacity instraw among rice varieties and growing locations as a potential source of natural bioactive compounds. Plants (Basel). 2022;11(21):2903. https://doi.org/10.3390/plants11212903
Lou Y, Joseph S, Li L, Graber ER, Liu X, Pan G. Water extract from straw biochar used for plant growth promotion: An initial test. Bio Res. 2016;11(1):249-66.https://doi.org/10.15376/biores.11.1.249-266
Gottimukkala KSV, Mishra B, Joshi S, Reddy MK. Cow urine: Plant growth enhancer and antimicrobial agent. J Hortic Plant Res. 2019;8:30-45. https://doi.org/10.18052/www.scipress.com/JHPR.8.30
Kumar R, Kaushik JK, Mohanty AK, Kumar S. Identification of bioactive components behind the antimicrobial activity of cow urine by peptide and metabolite profiling. Anim Biosci. 2023;36(7):1130-42. https://doi.org/10.5713%2Fab.22.0249
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