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Plant Science Today (PST)

Research Articles

Vol. 11 No. sp4 (2024): Recent Advances in Agriculture by Young Minds - I

Prevalence of groundnut root rot and antifungal potential of wild mushroom extracts against Macrophomina phaseolina

DOI
https://doi.org/10.14719/pst.5506
Submitted
3 October 2024
Published
24-12-2024 — Updated on 17-05-2025
Versions

Abstract

Macrophomina phaseolina is an important pathogen threatening groundnut production, with climate change induced variations in temperature and precipitation resulting in considerable yield reductions. This research provides a comprehensive evaluation of the root rot prevalence and severity in the primary groundnut-cultivating areas of Tamil Nadu. Findings indicate an incidence range of 32.71% to 85.66%. Pathogenic variability testing identified GMP-3 as the most virulent strain. Morphological differences and sporulation patterns were observed and M. phaseolina isolates were confirmed through ITS 1 (internal transcribed spacer 1) and ITS 4-sequencing, yielding Polymerase chain reaction amplicons of approximately 560 bp, which were submitted to Gene Bank. Additionally, a survey was conducted to collect various wild mushroom species from different regions in Tamil Nadu. Phenotypic and morphological characterization and molecular confirmation of the mushroom isolates were performed. The efficacy of these isolates against M. phaseolina was evaluated. The Tricholoma equestre (AWM-4) isolate demonstrated the highest inhibition in dual culture assays at 74.33%. Mushroom extracts prepared using various polar and nonpolar solvents were tested using the poisoned food technique to assess the inhibition percentage of M. phaseolina mycelial growth. Methanol extracts exhibited the greatest reduction in mycelial growth, with an inhibition rate of 75.56%. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the compounds with active antifungal properties.

References

  1. 1. FAOSTAT. World Agricultural Production (Rome, Italy: Food and Agriculture Organization of the United Nations) [Internet]. 2020. Available at: http://faostat.fao.org/
  2. 2. Pal KK, Singh R, Singh AL. Handbook of groundnut varieties of India released during 2001–2021. ICAR-Directorate of Groundnut Research; 2021.
  3. 3. Natarajan K, Hanif NAKA, Jayakumar J, Senguttuvan K, Gayathry G, Kumar BK, et al. A study on yield and value
  4. sustainability in groundnut (Arachis hypogea) through cluster frontline demonstrations approach in Cuddalore district of Tamil Nadu. Legume Res. 2024;47(7):1172-78. http://dx.doi.org/10.18805/LR-5292
  5. 4. Chakrabarty SK, Girish AG, Anitha K, Rao RD, Varaprasad KS, Khetarpal RK, Thakur RP. Detection, seedborne nature, disease transmission and eradication of seedborne infection by Rhizoctonia bataticola (Taub) butler in groundnut. Indian J Plant Prot. 2005;33(1):85-89.
  6. 5. Baird RE, Watson CE, Scruggs M. Relative longevity of Macrophomina phaseolina and associated mycobiota on
  7. residual soybean roots in soil. Plant Dis. 2003;87(5):563-66. https://doi.org/10.1094/pdis.2003.87.5.563
  8. 6. Reis FS, Martins A, Vasconcelos MH, Morales P, Ferreira ICFR. Functional foods based on extracts or compounds derived from mushrooms. Trends Food Sci Technol. 2017; 66:48-62. https://doi.org/10.1016/j.tifs.2017.05.010
  9. 7. Wong KH, Sabaratnam V, Abdullah N, Kuppusamy UR, Naidu M. Effects of cultivation techniques and processing on antimicrobial and antioxidant activities of Hericium erinaceus (Bull.: Fr.) Pers. extracts. Food Technol Biotechnol. 2009;47(1):47-55.
  10. 8. Niego AG, Rapior S, Thongklang N, Raspé O, Jaidee W, Lumyong S, Hyde KD. Macrofungi as a nutraceutical source: Promising bioactive compounds and market value. J Fungi. 2021;7(5):397. https://doi.org/10.3390/jof7050397
  11. 9. Muthukumar A, Naveenkunar R, Venkatesh A. Efficacy of water extracts of some mangrove plants for eco-friendly management of root rot disease of groundnut. J Plant Pathol Microb. 2014;5(5):1000243. http://dx.doi.org/10.4172/2157-7471.1000243
  12. 10. Rangaswami, Govindachetty, Mahadevan A. Diseases of crop plants in India: PHI Learning Pvt. Ltd; 1998.
  13. 11. White TJ, Bruns T, Lee SJ, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, white TJ, editors. PCR Protocols: A Guide to Methods and Applications. Academic press, Harcourt Brace Jovanovich, Publishers; 1990. p. 315-22. https://dx.doi.org/10.1016/B978-0-12-372180-8.50042-1
  14. 12. Tamura K, Stecher G, Kumar S. MEGA11: Molecular evolutionary genetics analysis version 11. Mol Biol Evol. 2021;38(7):3022-27. https://doi.org/10.1093/molbev/msab120
  15. 13. Tudses N. Isolation and mycelial growth of mushrooms on different yam-based culture media. J App Biol Biotech. 2016(5):33-36. https://dx.doi.org/10.7324/JABB.2016.40505
  16. 14. Ding XX, Xu X, Cui YY, Kost G, Wang PM, Yang ZL. A fifty-locus phylogenetic analysis provides deep insights into the phylogeny of Tricholoma (Tricholomataceae, Agaricales). Pers: Mol Phylogeny Evol Fungi. 2023;50:1-26. https://doi.org/10.3767/persoonia.2023.50.01
  17. 15. Dennis C, Webster J. Antagonistic properties of species-groups of Trichoderma: I. Production of non-volatile antibiotics. Trans Br Mycol Soc. 1971;57(1):25-39. https://doi.org/10.1016/S0007-1536(71)80077-3
  18. 16. Pandey KK, Pandey PK, Upadhyay JP. Selection of potential isolate of biocontrol agents based on biomass production, growth rate and antagonistic capability. Veg Sci. 2000;27(2):194-96.
  19. 17. Torres MJ, Brandan CP, Petroselli G, Erra-Balsells R, Audisio MC. Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds. Microbiol Res. 2016;182:31-39. https://doi.org/10.1016/j.micres.2015.09.005
  20. 18. Gebreyohannes G, Nyerere A, Bii C, DB Sbhatu. Determination of antimicrobial activity of extracts of indigenous wild mushrooms against pathogenic organisms. Evid Based Complement Alternat Med. 2019;2019:6212673. https://doi.org/10.1155/2019/6212673
  21. 19. Musharraf SG, Ahmed MA, Zehra N, Kabir N, Choudhary MI, Rahman AU. Biodiesel production from microalgal isolates of southern Pakistan and quantification of FAMEs by GC-MS/MS analysis. Chem Cent J. 2012;6:149. https://doi.org/10.1186/1752-153x-6-149
  22. 20. Okiti AF, Osuntokun OT. Antimicrobial, phytochemical analysis and molecular docking (in-silico approach) of Tithonia diversifolia (Hemsl.) A. Gray and Jatropha gossypiifolia L. on selected clinical and multi-drug resistant isolates. J Adv Microbiol. 2020;20(6):1-8. https://doi.org/10.9734/jamb/2020/v20i630248
  23. 21. Joseph J, Jeeva S. GC-MS and FT-IR analysis of a coastal medicinal plant- Hyptis suaveolens (L.) Poit. J Coast Life Med. 2016;4(5):380-85.
  24. 22. Dembitsky VM. Astonishing diversity of natural peroxides as potential therapeutic agents. J Mol Genet Med. 2015;9(1163):1747-862. https://doi.org/10.4172/1747-0862.1000163
  25. 23. Canpolat S, Canpolat EY. Antioxidant and antimicrobial activity of a medicinal mushroom, Ganoderma lucidum. J Adv Biol Biotechnol. 2023;26(11):60-67. https://doi.org/10.9734/jabb/2023/v26i11667
  26. 24. Liu M, He Y, Shen L, Anbari WHA, Li H, Wang J, et al. Asperteramide A, an unusual N-phenyl-carbamic acid methyl
  27. ester trimer isolated from the coral-derived fungus Aspergillus Terreus. Eur J Org Chem. 2019;(18):2928-32. https://
  28. doi.org/10.1002/ejoc.201900383
  29. 25. Kennedy GM, Min MY, Fitzgerald JF, Nguyen MT, Schultz SL, Crum MT, et al. Inactivation of the bacterial pathogens Staphylococcus pseudintermedius and Acinetobacter baumannii by butanoic acid. J Appl Microbiol. 2019;126(3):752-63. https://doi.org/10.1111/jam.14180
  30. 26. Chen Z, Liu Q, Zhao Z, Bai B, Sun Z, Cai L, et al. Effect of hydroxyl on antioxidant properties of 2, 3-dihydro-3, 5-dihydroxy-6-methyl-4 H-pyran-4-one to scavenge free radicals. RSC Adv. 2021;11(55):34456-61. https://doi.org/10.1039%2Fd1ra06317k
  31. 27. Muhammed D, Adebiyi YH, Odey BO, Alawode RA, Lawal A, Okunlola BM, et al. Dennettia tripetala (pepper fruit), a review of its ethno-medicinal use, phyto-constituents and biological properties. Gsc Adv Res Rev. 2021;6(3):035-43. https://doi.org/10.30574/gscarr.2021.6.3.0024
  32. 28. Gaur T, Rao PB. Analysis of antibacterial activity and bioactive compounds of the giant mushroom, Macrocybe gigantea (Agaricomycetes), from India. Int J Med Mushrooms. 2017;19(12):1083-1092. https://doi.org/10.1615/intjmedmushrooms.2017024559
  33. 29. Nkadimeng SM, Nabatanzi A, Steinmann CML, Eloff JN. Phytochemical, cytotoxicity, antioxidant and anti-inflammatory effects of Psilocybe natalensis magic mushroom. Plants. 2020;9(9):1127. https://doi.org/10.3390/plants9091127
  34. 30. Gandhi B, Greeshma K, Ruvulapalli DP, Kaki SS. Design, synthesis and antimicrobial evaluation of novel 10-undecenoic acid-based lipidic triazoles. Med Chem Res. 2022;31(9):1558-70. https://doi.org/10.1007/s00044-022-02940-9
  35. 31. Özkay Y, Tunali Y, Karaca H, Isikdag I. Antimicrobial activity of a new series of benzimidazole derivatives. Arch Pharm Res. 2011;34:1427-35. https://doi.org/10.1007/s12272-011-0903-8
  36. 32. Khan IH, Javaid A. Antifungal, antibacterial and antioxidant components of ethyl acetate extract of quinoa stem. Plant Prot. 2019;3(3):125-30. http://dx.doi.org/10.33804/pp.003.03.3109
  37. 33. Yahya Al-Ghamdi A. Phytochemical screening and in vitro antimicrobial potential of Aerva javanica leaf extracts, collected from Shada Mountain, Al-Baha, Saudi Arabia. Nov Res Microbiol J. 2022;6(2):1515-29. https://doi.org/10.21608/nrmj.2022.227886
  38. 34. Tabanca N, Demirci B, Crockett SL, Baser KHC, Wedge DE. Chemical composition and antifungal activity of Arnica
  39. longifolia, Aster hesperius and Chrysothamnus nauseosus essential oils. J Agric Food Chem. 2007;55(21):8430-35. https://doi.org/10.1021/jf071379
  40. 35. Guo J, Wang W, Hu J, Xie D, Gerhard E, Nisic M, et al. Synthesis and characterization of anti-bacterial and anti-fungal citratebased mussel-inspired bioadhesives. Biomaterials. 2016;85:204-17. https://doi.org/10.1016/j.biomaterials.2016.01.069
  41. 36. Singh P, Sharma A, Bordoloi M, Nandi SP. Antimicrobial, antioxidant, GC-MS analysis and molecular docking analysis of bioactive compounds of endophyte Aspergillus flavus from Argemone mexicana. J Microb Biotech Food Sci. 2023;13(1):e9970. http://dx.doi.org/10.55251/jmbfs.9970
  42. 37. Boucher MA, Côté H, Pichette A, Ripoll L, Legault J. Chemical composition and antibacterial activity of Tussilago farfara (L.) essential oil from Quebec, Canada. Nat Prod Res. 2020;34(4):545-48. https://doi.org/10.1080/14786419.2018.1489384
  43. 38. Ali A, Javaid A, Shoaib A. GC-MS analysis and antifungal activity of methanolic root extract of Chenopodium album against Sclerotium rolfsii. Planta Daninha. 2017;35:e017164713. https://doi.org/10.1590/S0100-83582017350100046
  44. 39. Shokrollahi N, Ho CL, Mohd Zainudin NAI, Abdul Wahab MAB, Wong MY. Plant defense inducers and antioxidant metabolites produced during oil palm-Ganoderma boninense interaction in vitro. Chemistry Africa. 2023;6:499-511. http://dx.doi.org/10.1007/s42250-022-00501-6
  45. 40. Veena GA, Padmodaya B, Devi RS, Manjula K, Naidu GM. Survey for disease incidence of groundnut collar rot, stem rot and root rot in Ananthapur, Kadapa, Chitttoor, Kurnool and Nellore districts of Andhra Pradesh. Int J Chem Stud. 2019;7(3):4932-4.
  46. 41. Pamala PJ, Jayalakshmi RS, Vemana K, Naidu GM, Varshney RK, Sudini HK. Prevalence of groundnut dry root rot (Macrophomina phaseolina (Tassi) Goid.) and its pathogenic variability in Southern India. Front Fungal Biol. 2023;4:1189043. https://doi.org/10.3389%2Fffunb.2023.1189043
  47. 42. Gade RM, Belkar YK, Ingle YV. Morphological and pathogenic variability among Rhizoctonia bataticola isolates associated with soybean (Glycine max L.) from India. Int J Curr Microbiol App Sci. 2018;7(1):2575-88. https://doi.org/10.20546/ijcmas.2018.701.310
  48. 43. Lakhran L, Ahir RR, Choudhary M, Choudhary S. Isolation, purification, identification and pathogenicity of Macrophomina phaseolina (Tassi) Goid caused dry root rot of chickpea. J Pharmacogn Phytochem. 2018;7(3):3314-17.
  49. 44. Almomani F, Alhawatema M, Hameed K. Detection, identification and morphological characteristic of Macrophomina phaseolina: The charcoal rot disease pathogens isolated from infected plants in Northern Jordan. Arch Phytopathol Plant Prot. 2013;46(9):1005-1014. https://doi.org/10.1080/03235408.2012.756174
  50. 45. Hoa NX, Trang LTH, Hieu TT, Loan LTT, Ngan LTM, Ho PT. Isolation and study on pure culture of wild edible mushrooms collected from provinces in the southeast region of Vietnam J Sci Technol. 2017;55(1A):122-33.
  51. 46. Thiribhuvanamala G, Prakasam V, Chandrasekar G, Sakthivel K, Veeralakshmi S, Velazhahan R, Kalaiselvi G. Biodiversity, conservation and utilization of mushroom flora from the Western Ghats region of India. In: Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7). 2011;155-64.
  52. 47. Imtiaj A, Jayasinghe C, Lee GW, Lee TS. Antibacterial and antifungal activities of Stereum ostrea, an inedible wild
  53. mushroom. Mycobiology. 2007;35(4):210-14. https://doi.org/10.4489%2FMYCO.2007.35.4.210
  54. 48. Luo DQ, Shao HJ, Zhu HJ, Liu JK. Activity in vitro and in vivo against plant pathogenic fungi of grifolin isolated from the basidiomycete Albatrellus dispansus. Z Naturforsch C. 2005;60(1-2):50-56. https://doi.org/10.1515/znc-2005-1-210
  55. 49. Rosa LH, Machado KMG, Jacob CC, Capelari M, Rosa CA, Zani CL. Screening of Brazilian basidiomycetes for antimicrobial activity. Mem Inst Oswaldo Cruz. 2003;98(7):967-74. http://dx.doi.org/10.1590/S0074-02762003000700019
  56. 50. Taskin H, Çelik ZD, Bozok F, Cabaroglu T, Büyükalaca S. First report on the volatile composition of Tricholoma anatolicum in comparison with Tricholoma caligatum. Rec Nat Prod. 2019;13(6):446-455. http://dx.doi.org/10.25135/rnp.122.18.12.1095
  57. 51. Maher RS, AL-Bayyar AH. Active compounds detection in aqueous extract of Ganoderma applanatum local isolate. Iraqi J Agric Sci. 2023;54(5):1273-78. http://dx.doi.org/10.36103/ijas.v54i5.1824

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