Optimization of ultrasound probe assisted extraction for PKM1 moringa seed oil
DOI:
https://doi.org/10.14719/pst.5566Keywords:
DPPH, FTIR, PKM1 moringa, moringa seed oil, total phenol content, Ultrasonic probe-assisted extractionAbstract
Moringa seed oil (MSO) was extracted from moringa seed powder (MSP) us- ing Soxhlet and ultrasound probe-assisted extraction (UPAE) methods. The process conditions for maximizing oil extraction were optimized using re- sponse surface methodology (RSM). The independent variables were 40- 60% ultrasound amplitude, 25-35 min of extraction time, and 1:10-1:20 of solvent-solute ratio for UPAE. The maximum oil yield obtained from the two extraction methods was 40.3 ± 0.79% for Soxhlet extraction (SE) and 36.32 ± 0.44% for UPAE. The process conditions for maximum extraction yield were 60% ultrasound amplitude, 29 min extraction time, and 1:19 of solvent-solute ratio for UPAE and at 60 °C of temperature, 6 h extraction time, and 300 mL of solvent for SE. The optimized extraction process has an oil recovery rate of 90.12% of the total oil content, with an iodine value of 69.18 ± 0.56 g I2/100 g, an acid value of 2.55 ± 0.02 mg KOH/g, and a peroxide value of 1.07 ± 0.02 meq O2/Kg. The physical characteristics of MSO included a refractive index of 1.467±0.03, a specific gravity of 0.92 ± 0.01, a pH of 6.12 ± 0.09, and colour values of 65.77 ± 0.34, 0.34 ± 0.01, and 61.2 ± 0.55 for L*, a* and b* respectively. The antioxidant activity and total phenol content (TPC) of UPAE oil were 33.6 ± 0.26% and 40.1 ± 0.67 ?g/mL, respectively.
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References
Sekhar C, Venkatesan N, Murugananthi D, Vidhyavathi A. Status of value addition and export of moringa produce in Tamil Nadu-a case study. International Journal of Horticulture. 2018;8. https://doi.org/10.5376/ijh.2018.08.0003
Chauhan Y. Global moringa oil market: By type (organic and conventional); application (pharmaceuticals, cosmetics, food and others); distribution channel (online, supermarket, retail stores, specialty stores and others); region-market size, industry dynamics, opportunity analysis and forecast for 2024-2032. New Delhi: Astute Analytica; 2024. [Available from: https://www.astuteanalytica.com/industry-report/moringa-oil-market.
Shahbaz M, Naeem H, Batool M, Imran M, Hussain M, Mujtaba A, et al. Antioxidant, anticancer and anti?inflammatory potential of Moringa seed and Moringa seed oil: A comprehensive approach. Food Science and Nutrition. 2024. https://doi.org/10.1002/fsn3.4312
Gharsallah K, Rezig L, B’chir F, Bourgou S, Achour NB, Jlassi C, et al. Composition and characterization of cold pressed Moringa oleifera seed oil. Journal of Oleo Science. 2022;71(9):1263-73. https://doi.org/10.5650/jos.ess22095
Kumar K, Srivastav S, Sharanagat VS. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review. Ultrasonics Sonochemistry. 2021;70:105325. https://doi.org/10.1016/j.ultsonch.2020.105325
Carreira-Casais A, Otero P, Garcia-Perez P, Garcia-Oliveira P, Pereira AG, Carpena M, et al. Benefits and drawbacks of ultrasound-assisted extraction for the recovery of bioactive compounds from marine algae. International Journal of Environmental Research and Public Health. 2021;18(17):9153. https://doi.org/10.3390/ijerph18179153
Esposito M, Piazza L. Ultrasound?assisted extraction of oil from hempseed (Cannabis sativa L.): Part 1. Journal of the Science of Food and Agriculture. 2022;102(2):732-39. https://doi.org/10.1002/jsfa.11404
Ladjouze B, ben Hamiche N, Kribeche A, Takka M, Boulekbache-Makhlouf L, Madani K.
Optimization of ultrasonic assisted extraction of oil from Moringa oleifera shelled and unshelled seeds. South African Journal of Botany. 2023;163:684-91. https://doi.org/10.1016/j.sajb.2023.11.004
Thirugnanasambandham K. Ultrasound-assisted extraction of oil from Moringa oleifera Lam. seed using various solvents. Energy Sources, Part A: Recovery, Utilization and Environmental Effects. 2018;40(3):343-50. https://doi.org/10.1080/15567036.2017.1416708
Zhong J, Wang Y, Yang R, Liu X, Yang Q, Qin X. The application of ultrasound and microwave to increase oil extraction from Moringa oleifera seeds. Industrial Crops and Products. 2018;120:1-10. https://doi.org/10.1016/j.indcrop.2018.04.028
Mohammadpour H, Sadrameli SM, Eslami F, Asoodeh A. Optimization of ultrasound-assisted extraction of Moringa peregrina oil with response surface methodology and comparison with Soxhlet method. Industrial Crops and Products. 2019;131:106-16. https://doi.org/10.1016/j.indcrop.2019.01.030
Ayerza R. Seed and oil yields of Moringa oleifera variety Periyakalum-1 introduced for oil production in four ecosystems of South America. Industrial Crops and Products. 2012;36(1):70-73. https://doi.org/10.1016/j.indcrop.2011.08.008
Leone A, Spada A, Battezzati A, Schiraldi A, Aristil J, Bertoli S. Moringa oleifera seeds and oil: Characteristics and uses for human health. International Journal of Molecular Sciences. 2016;17(12):2141. https://doi.org/10.3390/ijms17122141
Isopencu G, Stroescu M, Brosteanu A, Chira N, Pârvulescu OC, Busuioc C, et al. Optimization of ultrasound and microwave assisted oil extraction from sea buckthorn seeds by response surface methodology. Journal of Food Process Engineering. 2019;42(1):e12947. https://doi.org/10.1111/jfpe.12947
Zhao S, Zhang D. A parametric study of supercritical carbon dioxide extraction of oil from Moringa oleifera seeds using a response surface methodology. Separation and Purification Technology. 2013;113:9-17. https://doi.org/10.1016/j.seppur.2013.03.041
Hortwitz W, Latimer G. Official methods of analysis of AOAC Int. 2007.
Senrayan J, Venkatachalam S. Optimization of ultrasound-assisted solvent extraction (UASE) based on oil yield, antioxidant activity and evaluation of fatty acid composition and thermal stability of Coriandrum sativum L. seed oil. Food Science and Biotechnology. 2019;28:377-86. https://doi.org/10.1007/s10068-018-0467-1
Hamid F, Hamid FH. Manual of methods of analysis of foods. Food Safety and Standards Authority of India. 2015.
Ogbunugafor H, Eneh F, Ozumba A, Igwo-Ezikpe M, Okpuzor J, Igwilo I, et al. Physico-chemical and antioxidant properties of Moringa oleifera seed oil. Pakistan Journal of Nutrition. 2011;10(5):409-14. https://doi.org/10.3923/pjn.2011.409.414
Bhatnagar A, Krishna AG. Natural antioxidants of the Jaffna variety of Moringa Oleifera seed oil of Indian origin as compared to other vegetable oils. Grasas y Aceites. 2013;64(5):537-45. https://doi.org/10.3989/gya.010613
Oladipo B, Betiku E. Process optimization of solvent extraction of seed oil from Moringa oleifera: An appraisal of quantitative and qualitative process variables on oil quality using D-optimal design. Biocatalysis and Agricultural Biotechnology. 2019;20:101187. https://doi.org/10.1016/j.bcab.2019.101187
Yusoff MM, Gordon MH, Ezeh O, Niranjan K. Aqueous enzymatic extraction of Moringa oleifera oil. Food Chemistry. 2016;211:400-08. https://doi.org/10.1016/j.foodchem.2016.05.050
Mani S, Jaya S, Vadivambal R. Optimization of solvent extraction of Moringa (Moringa oleifera) seed kernel oil using response surface methodology. Food and Bioproducts Processing. 2007;85(4):328-35. https://doi.org/10.1205/fbp07075
Gharsallah K, Rezig L, Msaada K, Chalh A, Soltani T. Chemical composition and profile characterization of Moringa oleifera seed oil. South African Journal of Botany. 2021;137:475-82. https://doi.org/10.1016/j.sajb.2020.11.014
Raso J, Pagan R, Condon S, Sala F. Influence of temperature and pressure on the lethality of ultrasound. Applied and Environmental Microbiology. 1998;64(2):465-71. https://doi.org/10.1128/AEM.64.2.465-471.1998
Samaram S, Mirhosseini H, Tan CP, Ghazali HM, Bordbar S, Serjouie A. Optimisation of ultrasound-assisted extraction of oil from papaya seed by response surface methodology: Oil recovery, radical scavenging antioxidant activity and oxidation stability. Food Chemistry. 2015;172:7-17. https://doi.org/10.1016/j.foodchem.2014.08.068
Nde DB, Foncha AC. Optimization methods for the extraction of vegetable oils: A review. Processes. 2020;8(2):209. https://doi.org/10.3390/pr8020209
Hernández-Santos B, Rodríguez-Miranda J, Herman-Lara E, Torruco-Uco JG, Carmona-García R, Juárez-Barrientos JM, et al. Effect of oil extraction assisted by ultrasound on the physicochemical properties and fatty acid profile of pumpkin seed oil (Cucurbita pepo). Ultrasonics Sonochemistry. 2016;31:429-36. https://doi.org/10.1016/j.ultsonch.2016.01.029
Chen X, Li Z, Smith SA, Chen M, Liu H, Zhang J, et al. Optimization of supercritical CO2 extraction of Moringa oleifera seed oil using response surface methodological approach and its antioxidant activity. Frontiers in Nutrition. 2022;8:829146. https://doi.org/10.3389/fnut.2021.82914630.
Premi M, Sharma H. Oil extraction optimization and kinetics from Moringa oleifera (PKM 1) seeds. International Journal of Agriculture and Food Science Technology. 2013;4(4):371-78.
Dar IH, Junaid PM, Ahmad S, Shams R, Dash KK, Shaikh AM, et al. Optimization of ultrasound-assisted extraction of Nigella sativa seed oil for enhancement of yield and antioxidant activity. Discover Applied Sciences. 2024;6(3):104. https://doi.org/10.1007/s42452-024-05714-7
Moghimi M, Farzaneh V, Bakhshabadi H. The effect of ultrasound pretreatment on some selected physicochemical properties of black cumin (Nigella sativa). Nutrire. 2018;43(1):18. https://doi.org/10.1186/s41110-018-0077-y
Oroian M, Ursachi F, Dranca F. Influence of ultrasonic amplitude, temperature, time and solvent concentration on bioactive compounds extraction from propolis. Ultrasonics Sonochemistry. 2020;64:105021. https://doi.org/10.1016/j.ultsonch.2020.105021
Thilakarathna R, Siow LF, Tang T-K, Lee YY. A review on application of ultrasound and ultrasound assisted technology for seed oil extraction. Journal of Food Science and Technology. 2023;60(4):1222-36. https://doi.org/10.1007/s13197-022-05359-7
Bhimjiyani VH, Borugadda VB, Naik S, Dalai AK. Enrichment of flaxseed (Linum usitatissimum) oil with carotenoids of sea buckthorn pomace via ultrasound-assisted extraction technique: Enrichment of flaxseed oil with sea buckthorn. Current Research in Food Science. 2021;4:478-88. https://doi.org/10.1016/j.crfs.2021.07.006
Jamal AR, Kareem AA, editors. Use of ultrasound treatment to improve oil extraction from sunflower. IOP Conference Series: Earth and Environmental Science. IOP Publishing; 2022. https://doi.org/10.1088/1755-1315/1060/1/012081
Rahman N, Hashem S, Akther S, Jothi JS. Impact of various extraction methods on fatty acid profile, physicochemical properties and nutritional quality index of Pangus fish oil. Food Science and Nutrition. 2023;11(8):4688-99. https://doi.org/10.1002/fsn3.3431
Mehta N, Kumar P, Verma AK, Umaraw P, Khatkar SK, Khatkar AB, et al. Ultrasound-assisted extraction and the encapsulation of bioactive components for food applications. Foods. 2022;11(19):2973. https://doi.org/10.3390/foods11192973
Soares S, Rocha FR. Fast spectrophotometric determination of iodine value in biodiesel and vegetable oils. Journal of the Brazilian Chemical Society. 2018;29:1701-06. https://doi.org/10.21577/0103-5053.20180044
Zhang Q, Wu J, Ma P, Cai J, Zhang Y. Acid value determination and pre-esterification of crude Euphorbia lathyris L. oil. World Journal of Engineering and Technology. 2015;3(02):70. https://doi.org/10.4236/wjet.2015.32007
Wang X, Wang X, Wang T. An effective method for reducing free fatty acid content of high-acid rice bran oil by enzymatic amidation. Journal of Industrial and Engineering Chemistry. 2017;48:119-24. https://doi.org/10.1016/j.jiec.2016.12.028
Chemat F, Grondin I, Costes P, Moutoussamy L, Sing ASC, Smadja J. High power ultrasound effects on lipid oxidation of refined sunflower oil. Ultrasonics Sonochemistry. 2004;11(5):281-85. https://doi.org/10.1016/j.ultsonch.2003.07.004
Hosseini S, Gharachorloo M, Tarzi BG, Ghavami M, Bakhoda H. Effects of ultrasound amplitude on the physicochemical properties of some edible oils. Journal of the American Oil Chemists' Society. 2015;92(11-12):1717-24. https://doi.org/10.1007/s11746-015-2733-1
Ruslan SZ, Ahmad Zamanhuri N. Peroxide value and palm oil extraction yield from sterilised oil palm mesocarp. Malaysian Journal of Chemical Engineering and Technology (MJCET). 2023;6(2):144-51. https://doi.org/10.24191/mjcet.v6i2.22453
FAO W. Report of the 21st session of the codex alimentarius committee on fats and oils. Malaysia: Kola kinabala [Google Scholar]. 2009.
Tan SS. Papaya (Carica papaya L.) seed oil. Fruit Oils: Chemistry and Functionality. 2019;615-26. https://doi.org/10.1007/978-3-030-12473-1_31
Song J, Rezaee S, Guo W, Hernandez B, Puerto M, Vargas FM, et al. Evaluating physicochemical properties of crude oil as indicators of low-salinity–induced wettability alteration in carbonate minerals. Scientific Reports. 2020;10(1):3762. https://doi.org/10.1038/s41598-020-60106-2
Dinesha B, Nidoni U, Ramachandra C, Naik N, Sankalpa K. Effect of extraction methods on physicochemical, nutritional, antinutritional, antioxidant and antimicrobial activity of Moringa (Moringa oleifera Lam.) seed kernel oil. Journal of Applied and Natural Science. 2018;10(1):287-95. https://doi.org/10.31018/jans.v10i1.1619
Nebolisa NM, Umeyor CE, Ekpunobi UE, Umeyor IC, Okoye FB. Profiling the effects of microwave-assisted and soxhlet extraction techniques on the physicochemical attributes of Moringa oleifera seed oil and proteins. Oil Crop Science. 2023;8(1):16-26. https://doi.org/10.1016/j.ocsci.2023.02.003
Elsorady ME. Evaluation of Moringa oleifera seed oil extracted with different extraction methods. Croatian Journal of Food Science and Technology. 2023;15(1):1-7. https://doi.org/10.17508/CJFST.2023.15.1.01
Shapaval V, Afseth NK, Vogt G, Kohler A. Fourier transform infrared spectroscopy for the prediction of fatty acid profiles in Mucor fungi grown in media with different carbon sources. Microbial Cell Factories. 2014;13:1-11. https://doi.org/10.1186/1475-2859-13-86
Bhutada PR, Jadhav AJ, Pinjari DV, Nemade PR, Jain RD. Solvent assisted extraction of oil from Moringa oleifera Lam. seeds. Industrial Crops and Products. 2016;82:74-80. https://doi.org/10.1016/j.indcrop.2015.12.004
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