Physiological responses of sub1A QTL under induced dehydration stress for varying days in rice

Authors

  • Indraneel Saha Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani 741 235, West Bengal., India
  • Bipul Sarkar Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani 741 235, West Bengal., India
  • Arijit Ghosh Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani 741 235, West Bengal., India
  • Arnab Kumar De Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani 741 235, West Bengal., India
  • M K Adak Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani 741 235, West Bengal., India

DOI:

https://doi.org/10.14719/pst.2020.7.1.654

Keywords:

rice, sub1A QTL, physiological activity, reactive oxygen species, dehydration stress

Abstract

This study analysed the rice genotype with sub1A quantitative trait loci that may interact with ongoing exposure of dehydration. cv. Swarna Sub1 had more nutrients efficiency with increased membrane permeability than cv. Swarna. sub1A QTL with its expression to water / osmotic deficit, however, indirectly to impede the oxidative stress under dehydration might help cv. Swarna Sub1. At maximum dehydration cv. Swarna Sub1 had 1.12 fold excess electrolyte leakages than cv. Swarna under dehydration. cv. Swarna Sub1 had better Nicotinamide adenine dinucleotide phosphate-malic enzyme activity to secure carbon dioxide exchange. A proportional increase in enzyme activity all through dehydration stress maximize under light in cv. Swarna Sub1. At maximum dehydration cv. Swarna Sub1 at saturating substrate concentration was increased by 1.12 fold than other cultivar. The ratio of glutathione (GSH:GSSG) more depleted in cv. Swarna Sub1 through the dehydration period. cv. Swarna could be more promising to retrieve the activity by 1.80 fold than cv. Swarna Sub1 under maximum dehydration. Dehydroascorbate reductase activity was also maintained in cv. Swarna with 1.20 fold ahead than cv. Swarna Sub1 under same condition. As a biomarker of oxidative stress cv. Swarna Sub1 appeared to be less sensitive with the loss of protein oxidation, however, recorded with 25% less carbonyl content than cv. Swarna. Both the genotypes had scored a significant sensitivity with tissue specific distribution for reactive oxygen species as detected by histochemical assay.

Downloads

Download data is not yet available.

References

1. Ismail AM, Ella ES, Vergara GV, Mackill DJ. Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa). Annals of Botany. 2008; 103(2):197-209. https://doi.org/10.1093/aob/mcn211

2. Colmer TD, Armstrong W, Greenway H, Ismail AM, Kirk GJ, Atwell BJ. Physiological mechanisms of flooding tolerance in rice: transient complete submergence and prolonged standing water. In: Progress in Botany 2014 (pp. 255-307). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38797-5_9

3. Jackson MB, Ram PC. Physiological and molecular basis of susceptibility and tolerance of rice plants to complete submergence. Annals of Botany. 2003; 91(2):227-41. https://doi.org/10.1093/aob/mcf242

4. Singh US, Dar MH, SINGH S, Zaidi NW, Bari MA, Mackill DJ, Collard BC, Singh VN, Singh JP, Reddy JN, Singh RK. Field performance, dissemination, impact and tracking of submergence tolerant (Sub1) rice varieties in South Asia. SABRAO Journal of Breeding & Genetics. 2013; 45(1): 112-31.

5. Vijayan J, Senapati S, Ray S, Chakraborty K, Molla KA, Basak N, Pradhan B, Yeasmin L, Chattopadhyay K, Sarkar RK. Transcriptomic and physiological studies identify cues for germination stage oxygen deficiency tolerance in rice. Environmental and Experimental Botany. 2018; 147:234-48. https://doi.org/10.1016/j.envexpbot.2017.12.013

6. Sarkar RK, Bhattacharjee B. Rice genotypes with SUB1 QTL differ in submergence tolerance, elongation ability during submergence and re-generation growth at re-emergence. Rice. 2011; 5(1):7. https://doi.org/10.1007/s12284-011-9065-z

7. Alpuerto JB, Hussain RM, Fukao T. The key regulator of submergence tolerance, SUB1A, promotes photosynthetic and metabolic recovery from submergence damage in rice leaves. Plant, Cell & Environment. 2016;39(3):672-84. https://doi.org/10.1111/pce.12661

8. Jambunathan N. Determination and detection of reactive oxygen species (ROS), lipid peroxidation, and electrolyte leakage in plants. In: Plant Stress Tolerance 2010 (pp. 291-97). Humana Press. https://doi.org/10.1007/978-1-60761-702-0_18

9. Iglesias AA, Andreo CS. Purification of NADP-malic enzyme and phosphoenolpyruvate carboxylase from sugar cane leaves. Plant and Cell Physiology. 1989;30(3):399-405.

10. Kocsy G, Galiba G, Brunold C. Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants. Physiologia Plantarum. 2001;113(2):158-64. https://doi.org/10.1034/j.1399-3054.2001.1130202.x

11. Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology. 1981;22(5):867-80.

12. Verbeke P, Clark BF, Rattan SI. Modulating cellular aging in vitro: hormetic effects of repeated mild heat stress on protein oxidation and glycation. Experimental Gerontology. 2000;35(6-7):787-94. https://doi.org/10.1016/S0531-5565(00)00143-1

13. Li J, Pandeya D, Nath K, Zulfugarov IS, Yoo SC, Zhang H, Yoo JH, Cho SH, Koh HJ, Kim DS, Seo HS. ZEBRA?NECROSIS, a thylakoid?bound protein, is critical for the photoprotection of developing chloroplasts during early leaf development. The Plant Journal.2010;62(4):713-25. https://doi.org/10.1111/j.1365-313X.2010.04183.x

14. Flohe L. [10] Superoxide dismutase assays. In: Methods in enzymology 1984 (Vol. 105, pp. 93-104). Academic Press. https://doi.org/10.1016/S0076-6879(84)05013-8

15. Zeng N, Yang Z, Zhang Z, Hu L, Chen L. Comparative Transcriptome combined with proteome analyses revealed key factors involved in Alfalfa (Medicago sativa) response to waterlogging stress. International journal of Molecular Sciences. 2019;20(6):1359. https://doi.org/10.3390/ijms20061359

16. Saha I, Sarkar B, Ghosh A, De AK, Adak MK. Abscisic acid induced cellular responses of sub1A QTL to aluminium toxicity in rice (Oryza sativa L.). Ecotoxicology and Environmental Safety.2019;183: 109600. https://doi.org/10.1016/j.ecoenv.2019.109600

17. Saha I, De AK, Sarkar B, Ghosh A, Dey N, Adak MK. Cellular response of oxidative stress when sub1A QTL of rice receives water deficit stress. Plant Science Today. 2018; 5(3):84-94. https://doi.org/10.14719/pst.2018.5.3.387

18. Rizwan M, Mostofa MG, Ahmad MZ, Imtiaz M, Mehmood S, Adeel M, Dai Z, Li Z, Aziz O, Zhang Y, Tu S. Nitric oxide induces rice tolerance to excessive nickel by regulating nickel uptake, reactive oxygen species detoxification and defense-related gene expression. Chemosphere. 2018;191:23-35. https://doi.org/10.1016/j.chemosphere.2017.09.068

19. Spencer W, Bowes G. Photosynthesis and growth of water hyacinth under CO2 enrichment. Plant Physiology. 1986;82(2):528-33. https://doi.org/10.1104/pp.82.2.528

20. Omena-Garcia RP, Araújo WL, Gibon Y, Fernie AR, Nunes-Nesi A. Measurement of Tricarboxylic Acid Cycle Enzyme activities in plants. In: Plant Respiration and Internal Oxygen 2017; pp. 167-82. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7292-0_14

21. Rao X, Dixon RA. The differences between NAD-ME and NADP-ME subtypes of C4 photosynthesis: more than decarboxylating enzymes. Frontiers in Plant Science. 2016;7:1525. https://doi.org/10.3389/fpls.2016.01525

22. Murmu J, Chinthapalli B, Raghavendra AS. Light activation of NADP malic enzyme in leaves of maize: marginal increase in activity, but marked change in regulatory properties of enzyme. Journal of Plant Physiology. 2003;160(1):51-6.https://doi.org/10.1078/0176-1617-00844

23. de Carvalho Oliveira RA, de Andrade AS, Imparato DO, de Lima JG, de Almeida RV, Lima JP, de Bittencourt Pasquali MA, Dalmolin RJ. Analysis of Arabidopsis thaliana Redox Gene network indicates evolutionary expansion of class iii peroxidase in plants. Scientific Reports. 2019;9(1):1-9. https://doi.org/10.1038/s41598-019-52299-y

24. Mostofa MG, Hossain MA, Fujita M. Trehalose pretreatment induces salt tolerance in rice (Oryza sativa L.) seedlings: oxidative damage and co-induction of antioxidant defense and glyoxalase systems. Protoplasma. 2015;252(2):461-75. https://doi.org/10.1007/s00709-014-0691-3

25. Han B, Duan X, Wang Y, Zhu K, Zhang J, Wang R, Hu H, Qi F, Pan J, Yan Y, Shen W. Methane protects against polyethylene glycol-induced osmotic stress in maize by improving sugar and ascorbic acid metabolism. Scientific Reports. 2017;7:46185. https://doi.org/10.1038/srep46185

26. Luo FL, Nagel KA, Scharr H, Zeng B, Schurr U, Matsubara S. Recovery dynamics of growth, photosynthesis and carbohydrate accumulation after de-submergence: a comparison between two wetland plants showing escape and quiescence strategies. Annals of Botany. 2010;107(1):49-63. https://doi.org/10.1093/aob/mcq212

27. Liu Q, Zheng L, He F, Zhao FJ, Shen Z, Zheng L. Transcriptional and physiological analyses identify a regulatory role for hydrogen peroxide in the lignin biosynthesis of copper-stressed rice roots. Plant and Soil. 2015;387(1-2):323-36. https://doi.org/10.1007/s11104-014-2290-7

28. Saha I, De AK, Ghosh A, Sarkar B, Dey N, Adak MK. Preliminary Variations in Physiological Modules When sub1A QTL Is under Soil-Moisture Deficit Stress. American Journal of Plant Sciences. 2018; 9(04):732. https://doi.org/10.4236/ajps.2018.94058

29. Takahashi F, Suzuki T, Osakabe Y, Betsuyaku S, Kondo Y, Dohmae N, Fukuda H, Yamaguchi-Shinozaki K, Shinozaki K. A small peptide modulates stomatal control via abscisic acid in long-distance signalling. Nature. 2018;556(7700):235.https://doi.org/10.1038/s41586-018-0009-2

30. Zhang J, Zhang H, Srivastava AK, Pan Y, Bai J, Fang J, Shi H, Zhu JK. Knockdown of rice microRNA166 confers drought resistance by causing leaf rolling and altering stem xylem development. Plant physiology. 2018; 176(3):2082-94. https://doi.org/10.1104/pp.17.01432

Downloads

Published

06-02-2020

How to Cite

1.
Saha I, Sarkar B, Ghosh A, De AK, Adak MK. Physiological responses of sub1A QTL under induced dehydration stress for varying days in rice. Plant Sci. Today [Internet]. 2020 Feb. 6 [cited 2024 Nov. 21];7(1):112-21. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/654

Issue

Section

Research Articles