Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Vol. 7 No. 4 (2020)

Modalities of NADP-malic enzyme activities under light and darkness indicate its regulation with reference to C4 weed

DOI
https://doi.org/10.14719/pst.2020.7.4.754
Submitted
17 February 2020
Published
01-10-2020

Abstract

NADP –ME is the key enzyme for decarboxylation reactions in C4 CO2 concentration pathways. So, Amaranthus viridis has been evaluated with regards to photosynthetic NADP-malic enzyme for its response under light and darkness. Illumination (1000–1200 µEm-2s-1) for 40 minutes under 2 mM bicarbonate (HCO3-) sensitivity increased activity by 1.97 & 3.77-fold over darkness under 4.0 mM and 0.01 mM malate respectively. Limiting (0.01 mM) and saturated (4.0 mM) malate concentration had significant changes in enzyme activities. The different kinetic parameters indicated had the feedback inhibition under illumination. The activity with the inducer (citrate and succinate) and inhibitor (pyruvate and oxalate) was significant with substrate concentrations. Dithiol had reduced the activity by inhibition of the diminishing effect of light activation treatment. Therefore, NADP-ME is stringently regulated by redox changes with illumination as a key factor. Moreover, the pattern of polymorphic gene expression may be supportive in molecular modulation under light/darkness. This study may support the role of NADP-ME as a biomarker for C4 weed species under oxidative stress through light/darkness.

References

  1. Chen Q, Wang B, Ding H, Zhang J, Li S. The role of NADP-malic enzyme in plants under stress. Plant Science. 2019;281:206-12. https://doi.org/10.1016/j.plantsci.2019.01.010
  2. Amend JP, McCollom TM. Energetics of biomolecule synthesis on early Earth. ACS Publications. 2009;63-94.
  3. Drincovich MF, Casati P, Andreo CS. NADP?malic enzyme from plants: a ubiquitous enzyme involved in different metabolic pathways. FEBS letters. 2001;490(1-2):1-6. https://doi.org/10.1016/S0014-5793(00)02331-0
  4. Romanowska E, Buczy?ska A, Wasilewska W, Krupnik T, Dro?ak A, Rogowski P, Parys E, Zienkiewicz M. Differences in photosynthetic responses of NADP-ME type C4 species to high light. Planta. 2017; 245(3):641-57. https://doi.org/10.1007/s00425-016-2632-1
  5. De AK, Sarkar B, Saha I, Ghosh A, Dey N, Adak MK. Physiological characterisation of NADP-malic enzyme activity under 2,4-D toxicity in an aquatic fern, Azolla pinnata R.Br. African Journal of Plant Science. 2018; 12(9): 208-214. https://doi.org/10.5897/AJPS2018.1666
  6. Asami S, Inoue K, Matsumoto K, Murachi A, Akazawa T. NADP-malic enzyme from maize leaf: Purification and properties. Archives of Biochemistry and Biophysics. 1979; 194(2):503-10. https://doi.org/10.1016/0003-9861(79)90645-3
  7. Holaday AS, Lowder GW. Effect of pH on the Kinetic Parameters of NADP-Malic Enzyme from a C4Flaveria (Asteraceae) Species. Plant physiology. 1989; 90(2):401-05. https://doi.org/10.1104/pp.90.2.401
  8. Agarwal S, Sairam RK, Srivastava GC, Tyagi A, Meena RC. Role of ABA, salicylic acid, calcium and hydrogen peroxide on antioxidant enzymes induction in wheat seedlings. Plant Science. 2005;169(3):559-70. https://doi.org/10.1016/j.plantsci.2005.05.004
  9. Manivannan A, Soundararajan P, Halimah N, Ko CH, Jeong BR. Blue LED light enhances growth, phytochemical contents and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro. Horticulture, Environment and Biotechnology. 2015;56(1):105-13. https://doi.org/10.1007/s13580-015-0114-1
  10. Buczynska A, Wasilewska W, Drozak A, Romanowska E. Structural organisation of photosynthetic apparatus in mesophyll and bundle sheath chloroplasts of C4 plants of NADP-ME subtype. BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology. 2013;94(3):317-25.
  11. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. 1962;15(3):473-97.
  12. Force L, Critchley C, van Rensen JJ. New fluorescence parameters for monitoring photosynthesis in plants. Photosynthesis Research. 2003;78(1):17-33. https://doi.org/10.1023/A:1026012116709
  13. Murmu J, Chinthapalli B, Raghavendra AS. Light activation of NADP malic enzyme in leaves of maize: marginal increase in activity, but marked change in regulatory properties of enzyme. Journal of Plant Physiology. 2003;160(1):51-56. https://doi.org/10.1078/0176-1617-00844
  14. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry. 1976;72(1-2):248-54. https://doi.org/10.1016/0003-2697(76)90527-3
  15. Farooq M, Hussain M, Wahid A, Siddique KH. Drought stress in plants: an overview. In Plant responses to drought stress. Springer, Berlin, Heidelberg. 2012. p. 1-33. https://doi.org/10.1007/978-3-642-32653-0_1
  16. Hyskova VD, Miedzinska L, Dobra J, Vankova R, Ryalava H. Phosphoenolpyruvate carboxylase, NADP-malic enzyme and pyruvate, phosphate dikinase are involved in the acclimation of Nicotiana tabacum L. to drought stress. Journal of Plant Physiology. 2014;171(5):19-25. https://doi.org/10.1016/j.jplph.2013.10.017
  17. Patterson DT. Comparative ecophysiology of weeds. Weed Physiology: Volume I: Reproduction and Ecophysiology. 2018; 18:15.
  18. Moradi P, Ford-Lloyd B, Pritchard J. Metabolomic approach reveals the biochemical mechanisms underlying drought stress tolerance in thyme. Analytical Biochemistry. 2017;527:49-62. https://doi.org/10.1016/j.ab.2017.02.006
  19. Pocker Y, Miksch RR. Plant carbonic anhydrase. Properties and bicarbonate dehydration kinetics. Biochemistry. 1978; 17(6):1119-25.
  20. Bie Z, Ito T, Shinohara Y. Effects of sodium sulfate and sodium bicarbonate on the growth, gas exchange and mineral composition of lettuce. Scientia Horticulturae. 2004;99(3-4):215-24. https://doi.org/10.1016/S0304-4238(03)00106-7
  21. Wand SJ, Midgley GF, Stock WD. Growth responses to elevated CO2 in NADP-ME, NAD-ME and PCK C4 grasses and a C3 grass from South Africa. Functional Plant Biology. 2001; 28(1):13-25. https://doi.org/10.1071/PP99104
  22. Nayyar H, Gupta D. Differential sensitivity of C3 and C4 plants to water deficit stress: association with oxidative stress and antioxidants. Environmental and Experimental Botany. 2006; 58(1-3):106-13. https://doi.org/10.1016/j.envexpbot.2005.06.021
  23. Puga MI, Rojas-Triana M, de Lorenzo L, Leyva A, Rubio V, Paz-Ares J. Novel signals in the regulation of Pi starvation responses in plants: facts and promises. Current Opinion in Plant Biology. 2017;39:40-49. https://doi.org/10.1016/j.pbi.2017.05.007
  24. Pengelly JJ, Tan J, Furbank RT, von Caemmerer S. Antisense reduction of NADP-malic enzyme in Flaveria bidentis reduces flow of CO2 through the C4 cycle. Plant Physiology. 2012; 160(2):1070-80. https://doi.org/10.1104/pp.112.203240
  25. Ohnishi JI, Kanai R. Pyruvate uptake by mesophyll and bundle sheath chloroplasts of a C4 plant, Panicum miliaceum L. Plant and Cell Physiology. 1987;28(1):1-10. https://doi.org/10.1093/oxfordjournals.pcp.a077263
  26. Nakamoto H, Edwards GE. Effect of adenine nucleotides on the reaction catalysed by pyruvate, orthophosphate dikinase in maize. Biochemica et Biophysica Acta (BBA)-General Subjects. 1987; 924(2):360-68. https://doi.org/10.1016/0304-4165(87)90034-1
  27. Zhu XG, Wang Y, Ort DR, Long SP. e?photosynthesis: a comprehensive dynamic mechanistic model of C3 photosynthesis: from light capture to sucrose synthesis. Plant, Cell & Environment. 2013;36(9):1711-27. https://doi.org/10.1111/pce.12025
  28. Schaffer AA, Quick WP. Sucrose Metabolism in Sources and Sinks. In Photoassimilate Distribution Plants and Crops Source-Sink Relationships. Routledge, 2017. p. 115-58.
  29. Abogadallah GM. Differential regulation of photorespiratory gene expression by moderate and severe salt and drought stress in relation to oxidative stress. Plant Science. 2011; 180(3):540-47. https://doi.org/10.1016/j.plantsci.2010.12.004
  30. Busi R, Vila?Aiub MM, Beckie HJ, Gaines TA, Goggin DE, Kaundun SS, Lacoste M, Neve P, Nissen SJ, Norsworthy JK, Renton M. Herbicide?resistant weeds: from research and knowledge to future needs. Evolutionary Applications. 2013; 6(8):1218-21. https://doi.org/10.1111/eva.12098
  31. Doubnerová V, Ryšlavá H. What can enzymes of C4 photosynthesis do for C3 plants under stress? Plant Science. 2011;180(4):575-83. https://doi.org/10.1016/j.plantsci.2010.12.005
  32. Jiao Y, Lau OS, Deng XW. Light-regulated transcriptional networks in higher plants. Nature Reviews Genetics. 2007;8(3):217-30. https://doi.org/10.1038/nrg2049
  33. Mishra S, Joshi B, Dey P, Pathak H, Pandey N, Kohra A. CCM in photosynthetic bacteria and marine alga. Journal of Pharmacognosy and Phytochemistry. 2018;7(6):928-37.
  34. Bergamini CM, Gambetti S, Dondi A, Cervellati C. Oxygen, reactive oxygen species and tissue damage. Current Pharmaceutical Design. 2004;10(14):1611-26. https://doi.org/10.2174/1381612043384664
  35. Sarkar B, De AK, Saha I, Ghosh A, Debnath SC, Adak MK. Amelioration with titanium dioxide nanoparticle for regulation of oxidative stress in maize (Zea mays L.). The Journal of Microbiology, Biotechnology and Food Sciences. 2019;9(2):320-29. https://doi.org/10.15414/jmbfs.2019.9.2.320-329

Downloads

Download data is not yet available.