Evaluation of in vitro antioxidant and antidiabetic activities from Amomum nilgiricum leaf extract
DOI:
https://doi.org/10.14719/pst.2020.7.4.857Keywords:
Amomum nilgiricum, Antioxidant, Antidiabetic, Glucose diffusion, Glucose uptake, Insulin secretion, GC-MSAbstract
In the present study, hexane, ethyl acetate and methanol fraction of Amomum nilgiricum leaf was evaluated for antidiabetic efficacy, scavenging activities, followed by estimation of total phenol, total flavonoids. In the present study, a significant amounts of total phenolics (79.92±1.58 mg/g) and flavonoids (21.74± 0.89 mg/g) were showed from Ethyl acetae fraction. H2O2 scavenging activity (97.62±2.89%) with IC50 value of 78.57 µg/ml concentrations. Ethyl acetate fraction exhibited maximum inhibitory activity of glucose movement into outer solution across dialysis membrane at 250 µg/ml as compared to the control. The ethyl acetate fraction revealed maximum insulin secretory activity (130.5±3.66%) in RIN-m5F cells. Methanol fraction recorded maximum glucose uptake percent in yeast cells (67.08±1.68%) when compared to standard metronidazole (68.06±0.73%). This study scientifically validates the antidiabetic activity of A. nilgiricum. Hence, in view of its comparative hypoglycemic strength, it can work as a valuable healing agent in treating diabetes.
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Copyright (c) 2020 Narasimhamurthy Konappa, Udayashankar C Arakere, Soumya Krishnamurthy, Kusuma Chathrapalya Gangadharaiah, Vinod Gubbiveeranna, Nagaraju Shiviaha, Srinivas Chowdappa, Niranjana Siddapura
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