Differential expression of selected Arabidopsis resistant genes under abiotic stress conditions

Authors

DOI:

https://doi.org/10.14719/pst.2021.8.4.1213

Keywords:

Pathogen-associated molecular patterns, PAMP-triggered immunity, Effector triggered immunity Resistant genes, Decapping, Abiotic

Abstract

The plant immune system is equipped with several defensive layers to evade pathogen attack. One of the primary defense includes plasma membrane-localized receptors explicitly detect conserved pathogen-associated molecular patterns. Transcriptional reprogramming of resistant genes confers PAMP-triggered immunity. Consequently basal immunity is triggered which is primarily mediated by several intracellular nucleotide-binding leucine rich repeat receptors. Subsequently, nucleotide-binding leucine rich repeat receptors sense pathogens and activate another defense response known as effector triggered immunity. Both the PTI and ETI are mediated by resistant genes. Interestingly, the detailed molecular function of resistant genes is not yet fully revealed. Resistant genes are also well involved in non pathophysiological conditions such as during cold stress, heat stress, duration of exposure of light and drought stress. Here, we have reported that the Arabidopsis resistant genes AT1G17600, AT4G14368, AT4G16860, AT5G40910 and AT5G45050 are temperature regulated. We found that the transcript levels of AT1G58400, AT2G14080, AT2G17055, AT3G51560, AT4G16950, AT5G40910 and AT5G45050 were significantly raised for the plant samples grown under short-day conditions. The transcript levels of AT1G17600, AT1G27180, AT1G33560, AT2G14080, AT3G51560, AT4G16860 and AT4G16950 were upregulated for plants grown under drought stress conditions. In Arabidopsis, the transcriptional reprogramming is modulated by decapping protein factors. There was no significant change in the protein level of DCPs. Our results suggest that under abiotic stress conditions, the resistant genes differentially express independent of the decapping event.

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Author Biographies

Gagan Kumar Panigrahi, School of Applied Sciences, Centurion University of Technology and Management, Odisha 752 050, India

Assistant Professor, Centurion University of Technology and Management, Odisha, India

Annapurna Sahoo, School of Applied Sciences, Centurion University of Technology and Management, Odisha 752 050, India

Research Scholar, Centurion University of Technology and Management, Odisha, India

Kunja Bihari Satapathy, School of Applied Sciences, Centurion University of Technology and Management, Odisha 752 050, India

Emeritus Professor, Centurion University of Technology and Management, Odisha, India

References

Flor HH. Current status of the gene-for-gene concept. Annual Review of Phytopathology. 1971;9:275-96. https://doi.org/10.1146/annurev.py.09.090171.001423

Panigrahi GK, Satapathy KB. Sacrificed surveillance process favours plant defense: a review. Plant Archives. 2020;20(1):2551-59.

Böhm H, Albert I, Fan L, Reinhard A, Nürnberger T. Immune receptor complexes at the plant cell surface. Current Opinion in Plant Biology. 2014;20:47-54. https://doi.org/10.1016/j.pbi.2014.04.007

Macho AP, Zipfel C. Plant PRRs and the activation of innate immune signaling. Mol Cell. 2014;54(2):263-72. https://doi.org/10.1016/j.molcel.2014.03.028

Boller T, Felix G. A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annual Review of Plant Biology. 2009;60: 379-406. https://doi.org/10.1146/annurev.arplant.57.032905.105346

Panigrahi GK, Sahoo A, Satapathy KB. Insights to plant immunity: Defense signaling to epigenetics. Physiological and Molecular Plant Pathology. 2021;101568:1-7. https://doi.org/10.1016/j.pmpp.2020.101568

Panigrahi GK, Satapathy KB. Pseudomonas syringae pv. syringae infection orchestrates the fate of the Arabidopsis J domain containing cochaperone and decapping protein factor 5. Physiological and Molecular Plant Pathology. 2021;101598:1-9. https://doi.org/10.1016/j.pmpp.2020.101598

Jones JD, Dangl JL. The plant immune system. Nature. 2006;444:323-29. https://doi.org/10.1038/nature05286

Cui H, Tsuda K, Parker JE. Effector-triggered immunity: from pathogen perception to robust defense. Annual Review of Plant Biology. 2015;66:487-511. https://doi.org/10.1146/annurev-arplant-050213-040012

Dodds PN, Rathjen JP. Plant immunity: towards an integrated view of plant-pathogen interactions. Nature Reviews Genetics. 2010;11(8):539-48. https://doi.org/10.1038/nrg2812

Panigrahi GK, Sahoo AS. A review on Natural Dye: Gift from bacteria. International Journal of Bioassays. 2016;5(9):4909-12. https://doi.org/10.21746/ijbio.2016.12.0013

Panigrahi GK, Sahoo AS, Panda S. A complex network of molecular events triggered upon environmental cues which decide the fate of gene expression: a review. International Journal of Bioassays. 2016;5(12):5185-91. https://doi.org/10.21746/ijbio.2016.12.0013

Tsuda K, Glazebrook J, Katagiri F. The interplay between MAMP and SA signaling. Plant Signal Behav. 2008;3(6):359-61. https://doi.org/10.4161/psb.3.6.5702

Tsuda K, Sato M, Stoddard T, Glazebrook J, Katagiri F. Network properties of robust immunity in plants. PLOS Genetics. 2009;5(12):1-13. https://doi.org/10.1371/journal.pgen.1000772

Chisholm ST, Coaker G, Day B, Staskawicz BJ. Host-microbe interactions: shaping the evolution of the plant immune response. Cell. 2006;124(4):803-14. https://doi.org/10.1016/j.cell.2006.02.008

Karasov TL, Chae E, Herman JJ, Bergelson J. Mechanisms to mitigate the trade-off between growth and defense. Plant Cell. 2017;29:666-80. https://doi.org/10.1105/tpc.16.00931

Li X, Clarke JD, Zhang Y, Dong X. Activation of an EDS1-mediated R-gene pathway in the snc1 mutant leads to constitutive, NPR1-independent pathogen resistance. Mol Plant Microbe Interact. 2001;114:1131-39. https://doi.org/10.1094/MPMI.2001.14.10.1131

Maekawa T, Kufer TA, Schulze-Lefert P. NLR functions in plant and animal immune systems: so far and yet so close. Nat Immunol. 2011;12:817-26. https://doi.org/10.1038/ni.2083

Palma K, Thorgrimsen S, Malinovsky FG, Fiil BK, Nielsen HB, Brodersen P, Hofius D, Petersen M, Mundy J. Autoimmunity in Arabidopsis acd11 is mediated by epigenetic regulation of an immune receptor. PLoS Pathog. 2010;6:1-14. https://doi.org/10.1371/annotation/c70c3fcc-01df-4f98-aa4f-19697ccf7cfa

Shirano Y, Kachroo P, Shah J, Klessig DF. A gain of function mutation in an Arabidopsis Toll Interleukin1 receptor nucleotide binding site-leucine-rich repeat type R gene triggers defense responses and results in enhanced disease resistance. Plant Cell. 2002;l14:3149-62. https://doi.org/10.1105/tpc.005348

Halter T, Navarro L. Multilayer and interconnected post-transcriptional and co-transcriptional control of plant NLRs. Curr Opin Plant Biol. 2015;26:127–34. https://doi.org/10.1016/j.pbi.2015.06.014

Lai Y, Eulgem T. Transcript-level expression control of plant NLR genes. Mol Plant Pathol. 2017;19:1267-81. https://doi.org/10.1111/mpp.12607

Panigrahi GK, Satapathy KB. Arabidopsis DCP5, a decapping complex protein interacts with Ubiquitin-5 in the processing bodies. Plant Archives. 2020;20(1)2243-47.

Panigrahi GK, Satapathy KB. Formation of Arabidopsis Poly(A)-Specific Ribonuclease associated processing bodies in response to pathogenic infection. Plant Archives. 2020;20(2):4907-12.

Panigrahi GK, Sahoo SK, Sahoo A, Behera S, Sahu S, Dash A, Satapathy KB. Bioactive molecules from plants: a prospective approach to combat SARS-CoV-2. ADV TRADIT MED (ADTM). 2021;1-14. https://doi.org/10.1007/s13596-021-00599-y

Sahoo SK, Panigrahi GK, Sahoo A, Pradhan AK, Dalbehera A. Bio-hydrothermal synthesis of ZnO–ZnFe2O4 nanoparticles using Psidium guajava leaf extract: Role in waste water remediation and plant immunity. Journal of Cleaner Production. 2021; 128522: 1-13. https://doi.org/10.1016/j.jclepro.2021.128522

Published

21-08-2021 — Updated on 01-10-2021

How to Cite

1.
Panigrahi GK, Annapurna Sahoo, Satapathy KB. Differential expression of selected Arabidopsis resistant genes under abiotic stress conditions . Plant Sci. Today [Internet]. 2021 Oct. 1 [cited 2024 Nov. 4];8(4):859–864. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1213

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Research Articles