In vitro biological activity of seed oil of Withania coagulans and synthesis of catalyst from residual biomass

Priya Raghav; Nishesh  Sharma; Priyvart Choudhary; Nishant Kumar; Rohit  Sharma; Himani Badoni; Ayushi  Chaudhary

doi:10.14719/pst.3288

Authors

Priya Raghav Department of Biotechnology, School of Applied and Life sciences, Uttaranchal University, Dehradun- 248 007, Uttarakhand, India https://orcid.org/0000-0002-0594-6159
Nishesh Sharma Department of Biotechnology, School of Applied and Life sciences, Uttaranchal University, Dehradun- 248 007, Uttarakhand, India https://orcid.org/0000-0003-4350-5175
Priyvart Choudhary Research and Development Cell, Biotechnology Department, Manav Rachna International Institute of Research and Studies (Deemed to be University) Faridabad- 121 004, Haryana, India https://orcid.org/0000-0002-0047-6805
Nishant Kumar AAVYA Life science, Kanpur, Uttar Pradesh- 208 025, India https://orcid.org/0009-0004-9936-0950
Rohit Sharma Department of Food Science and Technology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand- 248 002, India https://orcid.org/0000-0001-9123-1063
Himani Badoni Department of Biotechnology, School of Applied and Life sciences, Uttaranchal University, Dehradun- 248 007, Uttarakhand, India https://orcid.org/0000-0002-6915-7263
Ayushi Chaudhary Department of Biotechnology, School of Applied and Life sciences, Uttaranchal University, Dehradun- 248 007, Uttarakhand, India https://orcid.org/0000-0001-5930-6387

DOI:

https://doi.org/10.14719/pst.3288

Keywords:

Withania coagulans, seeds, biocatalyst, XRD, SEM, EDX

Abstract

Withania coagulans is a well-known medicinal plant reported to possess numerous biological activities. However, the plant remains underutilized compared to other medicinal plants due to lack of clinical and analytical studies. In the present study, in vitro biological activities of oil extracted from seeds of W. coagulans were studied along with synthesis and characterization of catalyst from residue obtained post oil extraction. The extracted oil exhibited DPPH-radical scavenging activity, protein denaturation activity and in vitro -? amylase inhibitory activity. These activities were comparable to the corresponding activities of standard compounds indicating diverse medicinal potential of the oil. The residue obtained post oil extraction was utilized for synthesis of catalyst. The synthesized catalyst was characterized to identify the nature of the catalyst. Catalyst synthesized from residue was found to be silica-based catalyst, crystalline in nature and EDX mapping revealed catalyst to comprise of C, O, Na, Si, P and Ca. Interpretation of results obtained depict potential utilization of W. coagulans for diversified traditional as well as industrial application.

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