M4 generation of moringa (Moringa oleifera Lam.) mutants revealing genetic diversity for leaf traits based on SSR markers

Authors

  • S Bharathi Department of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Periyakulam, Theni 625 604, Tamil Nadu, India https://orcid.org/0009-0008-0334-9650
  • K Nageswari Department of Vegetable Science, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Periyakulam, Theni 625 604, Tamil Nadu, India https://orcid.org/0000-0002-6814-4233
  • S Rajesh Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India https://orcid.org/0000-0002-7000-8791
  • G Anand Department of Plant Breeding and Genetics, Krishi Vigyan Kendra, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625 104, Tamil Nadu, India https://orcid.org/0000-0002-9573-6410
  • P Geetharani Department of Seed Science and Technology, Agricultural Research Station, Vaigai Dam, Theni 625 512, Tamil Nadu Agricultural University, India https://orcid.org/0000-0002-6918-1757
  • J Rajangam Horticultural College and Research Institute, Tamil Nadu Agricultural University, Periyakulam, Theni 625 604, Tamil Nadu, India https://orcid.org/0000-0003-1292-1520
  • M Williams Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India https://orcid.org/0000-0002-1701-0681

DOI:

https://doi.org/10.14719/pst.4241

Keywords:

diversity, moringa, mutants, selection, SSR

Abstract

This study was conducted to develop a variety suitable for high leaf production in moringa. Initially, seeds of PKM 1 variety of moringa were treated with gamma irradiation of 100 Gy (Gray), 200 Gy and 300 Gy at Bhabha Atomic Research Centre (BARC), Mumbai and raised as M1 generation. The best-performing mutants were selected and forwarded to further generations (M1 to M4) based on leaf-related characters. A total of 10 Indian drumsticks (Moringa oleifera Lam.) mutants (derived from 200 Gy of gamma irradiation) from M4 generation with PKM 1 variety were evaluated for morphological and molecular diversity. To evaluate morphological diversity, 4 features (leaf length, leaf breadth, fresh weight and dry weight) were studied under field conditions. Ten sets of markers for simple sequence repeats (SSR), i.e. GenicSSR1081, SSR25987, SSR2927, GenicSSR859, GenicSSR796, SSR6561, GenicSSR890, GenicSSR1204, GenicSSR983 and GenicSSR778; that generated distinct and repeatable bands; were chosen for analyzing molecular diversity. A total of 24 bands were amplified, of which 17 were polymorphic. With an average of 0.391, the polymorphic information content (PIC) values for SSR markers varied from 0.124 for marker SSR2927 to 0.719 for marker GenicSSR778. Based on the cluster analysis with SSR markers, the mutants are divided into 2 groups. The variety PKM 1 was separated by a group whereas; all the other mutants fell into a single group indicating the diversity of mutants from the control. The diverse mutants (15-1-09-70 and 15-1-09-53) based on both morphological and molecular observations were selected and suggested for evaluation through subsequent generations that have the potential to be released as a variety for high leaf production. By increasing the production of highly nutritious moringa leaf, self-sufficiency and nutritional security can be obtained as well as it could benefit the lifestyle improvement of farmers through export to countries where the crop demand is high.

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References

Suzana D, Suyatna FD, Andrajati R, Sari SP, Mun’im A. Effect of Moringa oleifera leaves extract against hematology and blood biochemical value of patients with iron deficiency anemia. J of Young Pharmacists. 2017;9(1):79. https://doi.org/10.5530jyp.2017.1s.20

Cretella ABM, da Silva SB, Pawloski PL, Ruziska RM, Scharf DR, Ascari J, et al. Expanding the anti-inflammatory potential of Moringa oleifera: topical effect of seed oil on skin inflammation and hyperproliferation. J Ethnopharmacol. 2020;254:112708. https://doi.org/10.1016/j.jep.2020.112708

Mehta A, Agrawal B. Investigation into the mechanism of action of Moringa oleifera for its anti-asthmatic activity. Orient Pharm Exp Med. 2008;8(1):24–31. https://doi.org/10.3742/OPEM.2008.8.1.024

Popoola JO, Obembe OO. Local knowledge, use pattern and geographical distribution of Moringa oleifera Lam. (Moringaceae) in Nigeria. J Ethnopharmacol. 2013;150(2):682–91. https://doi.org/10.1016/j.jep.2013.09.043

Saleem A, Saleem M, Akhtar MF. Antioxidant, anti-inflammatory and antiarthritic potential of Moringa oleifera Lam: An ethnomedicinal plant of Moringaceae family. South African J Bot. 2020;128:246–56. https://doi.org/10.1016/j.sajb.2019.11.023

Abdulrahman MD, Hamad SW. Traditional methods for treatment and management of measles in northern Nigeria: Medicinal plants and their molecular docking. Ethnobot Res and Appl. 2022;23. http://eprints.tiu.edu.iq/id/eprint/991

Sanjay P, Dwivedi K, Shigru. (Moringa oleifera Lam.): A critical review. Int J Ayu Pharm Chem. 2015;3.

Milla PG, Penalver R, Nieto G. Health benefits of uses and applications of Moringa oleifera in bakery products. Plants. 2021;10(2):318. https://doi.org/10.3390/plants10020318

Pandit R, Bhusal B, Regmi R, Neupane P, Bhattarai K, Maharjan B, et al. Mutation breeding for crop improvement: A review. Revin Food and Agri. 2021;2(1):31–35. http://doi.org/10.26480/rfna.01.2021.31.35

Yali W, Mitiku T. Mutation breeding and its importance in modern plant breeding. J Plant Sci. 2022;10(2):64–70. http://doi.org/10.11648/j.jps.20221002.13

He C, Poysa V, Yu K. Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars. Theor Appl Genet. 2003;106:363–73. http://doi.org/10.1007/s00122-002-1076-0

Park YJ, Lee JK, Kim NS. Simple sequence repeat polymorphisms (SSRPs) for evaluation of molecular diversity and germplasms classification of minor crops. Mol. 2009;14:4546–69. http://doi.org/10.3390/molecules14114546

Wu JC, Yang J, Gu ZJ, Zhang YP. Isolation and characterization of twenty polymorphic microsatellite loci for Moringa oleifera (Moringaceae). Hort Sci. 2010;45:690–92.

Popoola JO, Bello OA, Olugbuyiro JA, Obembe OO. Simple sequence repeats (SSR) analysis of genetic intraspecific relationships of Moringa oleifera populations from Nigeria. J critical rev. 2017;9(1): https://doi.org/10.21273/HORTSCI.45.4.690

Zheng X, Cheng T, Yang L, Xu J, Tang J, Xie K. Genetic diversity and DNA fingerprints of three important aquatic vegetables by EST-SSR markers. Scientific Reports. 2019;9(1):1–10. https://doi.org/10.1038/s41598-019-50569-3

Udhaya KD. Induced polygenic variability in PKM 1 moringa variety for enhanced leaf biomass production. Int J Agri Sci. 2019;975–3710. https://www.bioinfopublication.org/jouarchive.php?opt=&jouid=BPJ0000217

Hari K, Nageswari K, Janavi GJ, Geetharani P. Genetic variability, correlation and path analysis of M3 generation mutants in moringa (Moringa oleifera L.) for leaf biomass. Biol Forum Int J. 2022;14(3):135–42.

Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 1987;19:11–15.

Mgendi MG. Genetic diversity between cultivated and non-cultivated Moringa oleifera Lam. Provenances assessed by RAPD markers. J Cell Mol Biol. 2010;8(2):95. http://jcmb.halic.edu.tr

Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. PNAS USA. 1979;76:5269–73. https://doi.org/10.1073/pnas.76.10.526

Botstein D, White RL, Skalnick MH, Davies RW. Construction of a genetic linkage map in man using restriction fragment length polymorphism. American J Human Gen. 1980;32:314–31. https://pmc.ncbi.nlm.nih.gov/articles/PMC1686077

Chesnokov YV, Artemyeva AM. Evaluation of the measure of polymorphism information of genetic diversity. Agri Biol. 2015;50(5):571–78.

Boopathi MN, Williams M, Ranjani VR, Paul AE, Jayakanthan M, Saranya N, et al. Development of novel SSR markers derived from genomic and transcriptomic data of Moringa oleifera L. var. PKM 1 and their applicability. The J Horti Sci Biotech. 2022;97(4):487–95. https://doi.org/10.1080/14620316.2021.2014991

Kpocheme AOEK, Hotegni NF, Missihoun AA, Gnanvi BN, Atou R, Wouyou A, et al. Morphological characterization of Amaranthus cruentus L. mutant lines derived from local and preferred Amaranthus cultivar. Int J Biol Chem Sci. 2022;16(4):1554–69. https://doi.org/10.4314/ijbcs.v16i4.16

Mansour HM, Hamideldin N, Abdel-Tawab FM, Fahmy EM, El Demerdash H, Amar MH. A physiological and genetical study for the effect of gamma irradiation on Moringa olefiera Lam. Egyptian J Radi Sci Appli. 2018;31(2):125–36.

Ashalata VA, Kotasthane AS. Effect of gamma radiation treatment on growth and morphology of red Amaranthus. The Pharma Innov J. 2022;SP-11(9):1469–71. https://doi.org/10.21608/ejrsa.2018.2286.1035

Nandhini V, Boopathi NM, Raveendran M, Rajasree V. Enhancing moringa (Moringa oleifera) leaf biomass using chemical induced mutagenesis and validating the mutagenesis using simple sequence repeats. Survival. 2022;10:100.

Hassanein AMA. Nutritional, chemical and molecular characterisation of Moringa oleifera Lam. and Moringa peregrina (Forssk.) Fiori genotypes. The J Horti Sci Biotech. 2018;93(5):500–09. https://doi.org/10.1080/14620316.2017.1399834

Saraswathi T, Meena P, Pugalendhi L, Boopathi NM. Assessment of genetic divergence and molecular characterization of moringa (Moringa oleifera Lam.) genotypes. South African J of Bot. 2023;157:502–07. https://doi.org/10.1016/j.sajb.2023.04.020

Haque MI, Ishtiaque S, Islam MM, Mujahidi TA, Rahim MA. Molecular profiling of chilli germplasm by using SSR marker. SAARC J of Agri. 2021;19(1):1–13. https://doi.org/10.3329/sja.v19i1.54774

Published

03-03-2025 — Updated on 01-04-2025

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1.
Bharathi S, Nageswari K, Rajesh S, Anand G, Geetharani P, Rajangam J, Williams M. M4 generation of moringa (Moringa oleifera Lam.) mutants revealing genetic diversity for leaf traits based on SSR markers. Plant Sci. Today [Internet]. 2025 Apr. 1 [cited 2025 Apr. 3];12(2). Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/4241

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