Forthcoming

Effect of gamma rays induced mutagenesis on the agronomical and biochemical traits of acid lime

Authors

DOI:

https://doi.org/10.14719/pst.4120

Keywords:

Gamma irradiation, lethal dose, PKM 1, survival and mortality

Abstract

One of the indigenous citrus crops of India is acid lime, which has a high economic value and unique flavour compared to other citrus fruits. The current study was intended to ascertain the gamma ray dosage that proves lethal for the acid lime variety PKM1. The acid lime seeds were exposed to nine distinct gamma radiation dosages ranging from 5 to 45 Gy with an interval of 5 Gy and compared to untreated control. Different irradiation doses demonstrated substantial differences in morphological and biochemical traits. Increased dosage of gamma radiation leads to significant changes in various agronomic parameters of plant germination, number of plants, plant height, and change in biochemical traits. Various gamma radiation doses revealed a low survival rate after 35 Gy, with LD 50 at 27.86 Gy.

Downloads

Download data is not yet available.

References

Maheswari KU, Prathap B, Latha PM. Flowering pattern and fruiting behaviour in acid lime (Citrus aurantifolia Swingle). The Pharma Innovation Journal. 2023;12(4):2733-35.

Rajamanickam C, Sundaraiya K, Muralidharan B, Baskaran A, Sumathi T. Evaluation of different citrus rootstocks on growth characters of acid lime (Citrus aurantifolia Swingle) var. PKM-1. The Pharma Innovation Journal. 2023;12(3):5842-44.

Jo Y, Ameer K, Chung N, Kwon JH. Influence of gamma irradiation on volatile flavour profiles and physicochemical attributes of navel oranges (Citrus sinensis L.) under post-irradiation storage. International Food Research Journal. 2019;26(3):1035-43.

Kurbett FK, Khyadagi K. Quality attributes and nutrient composition of acid lime varieties. The Pharma Innovation Journal. 2023;12(11):384-88. https://doi.org/10.22271/tpi.2023.v12.i11e.23870

Kumar NC, Rajangam J, Balakrishnan K, Kavya MV. Influence of foliar application of micronutrients on yield and quality of mandarin orange (Citrus reticulata Blanco.) under lower pulney hills. International Journal of Agriculture Sciences. 2017;9(17):4151-53.

Ladaniya MS, Marathe RA, Das AK, Rao CN, Huchche A, Shirgure PS, Murkute AA. High density planting studies in acid lime (Citrus aurantifolia Swingle). Scientia Horticulturae. 2020;261:108935. doi.org/10.1016/j.scienta.2019.108935

Mbaye G, Soumboundou M, Diouf L, Ndong B, Djiboune A, Sy P, et al. Evaluation of the effects of irradiation of peanut grain by a gamma-ray beam on culture. Open Journal of Biophysics. 2017;7:94-100. doi: 10.4236/ojbiphy.2017.73008

Sutarto I, Agisimanto D, Supriyanto A. Development of promising seedless Citrus mutants through gamma irradiation. Q.Y. Shu (ed.), Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations, Rome. 2009; 306-08.

Goldenberg L, Yaniv Y, Porat R, Carmi N. Effects of gamma-irradiation mutagenesis for induction of seedlessness, on the quality of mandarin fruit. Food and Nutrition Sciences. 2014;5:943-52. https://doi.org/10.4236/fns.2014.510105

Purba DP, Husni A, Akhidaya A, Kosmiatin M, Purwito A. Effect of gamma rays irradiation and in vitro selection on Citrus nobilis (L.) ‘Siam Banyuwangi’ to huanglongbing (HLB) disease. AGRIVITA Journal of Agricultural Science. 2021;43(2):358-66. https://doi.org/10.17503/agrivita.v43i2.2887

Kumar PP, Madhumathi C, Madhavi M, Priya BT, Rao MP, Kavitha M, Naik EK. Mutagenic impacts and LD50 determination in acid lime (Citrus aurantifolia) Cv. Petlur acid lime. The Pharma Innovation Journal. 2023;12(11): 661-64.

Khalil SA, Sattar A, Zamir R. Development of sparse-seeded mutant kinnow (Citrus reticulata Blanco) through budwood irradiation. African Journal of Biotechnology. 2011;10(65):14562-65. https://doi.org/10.5897/AJB10.1810

Eun, CH, Kim, IJ. The Citrus mutant Jedae-unshiu induced by gamma irradiation exhibits a unique fruit shape and increased flavonoid content. Plants. 2022;11:1337. https://doi.org/10.3390/ plants11101337

Rattanpal HS, Singh G, Gupta M. Studies on mutation breeding in mandarin variety Kinnow. Current Science. 2019;116(3):483-83. https://doi.org/10.18520/cs/v116/i3/483-487

Mallick M, Awasthi OP, Singh SK, Dubey AK. Physiological and biochemical changes in pre-bearing mutants of Kinnow mandarin (C. nobilis Lour × C. deliciosa Tenora). Scientia Horticulturae. 2016;199:178-85. https://doi.org/10.1016/j.scienta.2015.12.050

Jain SM. Mutagenesis in crop improvement under the climate change. Romanian Biotechnological Letters. 2010;15(2):88-106.

Maan SS, Brar JS. Mutagenic sensitivity analysis in guava (Psidium gujava L.). Fruits. 2021;76(4):181-90. doi.org/10.17660/th2021/76.4.3.

Piri I, Babayan M, Tavassoli A, Javaheri M. The use of gamma irradiation in Agriculture. African Journal of Microbiology Research. 2011;5(32):5806-11. https://doi.org/10.5897/AJMR11.949

Barr HD, Weatherley PE. A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust J Biol Sci. 1962;15:413-28. https://doi.org/10.1071/BI9620413

Porra RK, Thompson WA, Kriedemann PE. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophyll a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectrometry. Biochem Biophys Acta J. 1989;975(3):384-94. https://doi.org/10.1016/S0005-2728(89)80347-0

Sadasivam S, Manickam A. Biochemical methods for agricultural sciences. Wiley Eastern Limited New Delhi; 1992.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J biol Chem. 1951;193(1):265-75. https://doi.org/10.1016/S0021-9258(19)52451-6

Saini HK, Gill MIS. Induction of mutation in Rough lemon (Citrus jambhiri Lush.) using gamma rays. J Hortl Sci Vol. 2009;4(1):41-44. https://doi.org/10.24154/jhs.v4i1.554

Ahloowalia BS. In vitro techniques and mutagenesis for the improvement of vegetatively propagated plants. Current Plant Science and Biotechnology in Agriculture. 1998;32:293-309. https://doi.org/10.1007/978-94-015-9125-6_15

Rakshi M, Kavitha C, Pugalendhi L, Paramaguru P, Sudhakar D, Ganesan NM Soorianathasundaram K. Determination of Radio Sensitivity (LD50) of embryo cultured of banana (Musa balbisiana) cv. Bhimkol. International Journal of Environment and Climate Change. 2022; 12(11):2432-40. DOI: 10.9734/IJECC/2022/v12i1131236

Abdulhafiz F, Kayat F, Zakaria S. Effect of gamma irradiation on the morphological and physiological variation from in vitro individual shoot of banana cv. Tanduk (Musa spp.). J Plant Biotechnol. 2018;45:140-45. https://doi:org/10.5010/JPB.2018.45.2.140

Mahadevamma M, Dinesh MR, Kumari RV, Shankarappa TH. Evaluation of induced variability in papaya (Carica papaya L.) by physical mutagenesis. CIBTech Journal of Biotechnology. 2012;1(1):66-71.

Islam AF, Islam MM, Hasan MM. Effect of gamma irradiation doses on morphological and biochemical attributes of grape saplings. Agricultural Sciences. 2015;6:505-12. http://dx.doi.org/10.4236/as.2015.65050

Kok D. Involvement of peroxidase activity in various sensitivity to gamma irradiation in scions of Cabernet Sauvignon and Merlot cvs (Vitis vinifera L.). Journal of Food, Agriculture and Environment. 2011;9(2):392-96.

Saadati S, Azam B, Rahemi MR, Khiabani BN. Alteration of physiological and biochemical properties in leaves and fruits of pomegranate in response to gamma irradiation. Scientific Reports. 2022;12:4312. Doi: 10.1038/s41598-022-08285-y

Ling APK, Chia JY, Hussein S, Harun AR. Physiological responses of Citrus sinensis to gamma irradiation. World Appl Sci J. 2008;5(1):12-19.

Taha RA, El-Nagdi WMA, Hedaya AK. Micropropagated banana plants induced by gamma irradiation and resistant to the root-knot nematode reproduction. Agricultural Engineering International: CIGR Journal. 2020;22(2):217-25.

Devi DM, Ranjithkumar SC, Rajangam C, Santha S, Sankar C. Determination of lethal dose (LD50) and effect of physical and chemical mutagenesis in acid lime var. PKM 1. The Pharma Innovation Journal. 2021;10(11):583-88.

Published

04-12-2024

How to Cite

1.
Manjusha M, Rajangam J, Saraswathy S, Venkatesan K, Rajesh S, Madhan Mohan M, Ganasekaran M. Effect of gamma rays induced mutagenesis on the agronomical and biochemical traits of acid lime. Plant Sci. Today [Internet]. 2024 Dec. 4 [cited 2024 Dec. 22];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/4120

Issue

Section

Research Articles

Most read articles by the same author(s)