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Plant Science Today (PST)

Research Articles

Early Access

Sun protection factor value of Rosa centifolia and Rosa alba from Merapi mountain Indonesian National Park and its potency as sunscreen

DOI
https://doi.org/10.14719/pst.4916
Submitted
1 September 2024
Published
11-02-2025
Versions

Abstract

The potential wild Rosa centifolia L. and Rosa alba extracts in the Sapuangin region of Merapi Mountain National Park have not been explored. This study investigated the phytochemical content, antioxidant activity, and potential of extracts from R. centifolia and R. alba as sunscreen. R. centifolia and R. alba simplicia were extracted with methanol and ethanol to obtain four rose extract types. Phytochemical content was investigated using qualitative assay. Spectrophotometry was used to determine total flavonoid (TFC) and phenolic content (TPC). Antioxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Potential extracts as sunscreen were evaluated with Sun Protection Factor (SPF) assay. The qualitative phytochemical assay showed both ethanolic and methanolic extracts of R. centifolia contained saponin, tannin, flavonoid, and triterpenoid. Alkaloids were only contained in the methanolic extracts of R. centifolia. Ethanolic and methanolic extract of R. alba contained saponin and flavonoid. Alkaloid was present in the ethanolic extract of R. alba, while tannin is only in the methanolic extract of R. alba. The highest TFC was shown by methanolic extract of R. alba (68.132 ± 0.484 mg /QE/g). Methanolic extract of R. centifolia showed the highest TPC (253.090±2.530 mg GAE/g). Methanolic extract of R. centifolia exhibited IC50 values of DPPH scavenging 22,622 µg/mL and was categorized as a strong antioxidant activity. Likewise, the SPF value is the highest among the other extracts, namely 32.602 ± 0.430 at a concentration of 500 ppm. R. centifolia and R. alba ethanolic and methanolic extracts have the potential to be sued as candidates for antioxidant and sunscreen sources.

References

  1. Lim KM. Skin epidermis and barrier function. Int J Mol Sci. 2021;22(6):3035. https://doi.org/10.3390/ijms22063035
  2. Gasparro FP, Mitchnick M, Nash JF. A review of sunscreen safety and efficacy. J Photochem Photobiol. 1998;68(3):243–56. https://doi.org/10.1111/j.1751-1097.1998.tb09677.x
  3. Biniek K, Levi K, Dauskardt RH. Solar UV radiation reduces the barrier function of human skin. Proc Natl Acad Sci. 2012;109(42):17111–16. https://doi.org/10.1073/pnas.1206851109
  4. Wimpy, Harningsih T, Larasati WT. Uji aktivitas antioksidan dan tabir surya kombinasi ekstrak kulit buah pisang kepok (Musa paradisiaca Linn.) dan ekstrak kulit buah alpukat (Persea americana Mill.). J Ilmiah Manuntung. 2020;6(2):231–39. https://doi.org/10.51352/jim.v6i2.365
  5. Thallaj N, Labban L. The medicinal and pharmacological properties of Damascene rose (Rosa damascena): A review. Int J Herb Med. 2020;8(2):33-37.
  6. Jitendra J, Vineeta T, Ashok K, Brijesh K, Singh P. Rosa centifolia: Plant review. Int J Res Pharm Chem. 2012;2(3):794-96.
  7. Tatke P, Satyapal US, Mahajan DC, Naharwar V. Phytochemical analysis, in-vitro antioxidant and antimicrobial activities of flower petals of Rosa damascena. Int J Pharmacogn Pharm Res. 2015;7(2):246-50.
  8. Verma A, Srivastava R, Sonar PK, Yadav R. Traditional, phytochemical and biological aspects of Rosa alba L.: A systematic review. Fut J Pharma Sci. 2020;6(1). https://doi.org/10.1186/s43094-020-00132-z
  9. Georgieva A, Dobreva A, Tzvetanova E, Alexandrova A, Mileva M. Comparative study of phytochemical profiles and antioxidant properties of hydrosols from Bulgarian Rosa alba L. and Rosa damascena Mill. J Essent Oil Bear Plants. 2019;22(5):1362-71. https://doi.org/10.1080/0972060X.2019.1699867
  10. Alom S, Ali F, Bezbaruah R, Kakoti BB. Rosa alba linn.: A comprehensive review on plant profile, phytochemistry, traditional and pharmacological uses. World J Pharm Res. 2021;10(4):798-811. https://doi.org/10.20959/wjpr20214-20135
  11. Handayani TW, Yusuf Y, Tandi J. Analisis kualitatif dan kuantitatif metabolit sekunder ekstrak biji kelor (Moringa oleifera Lam.) dengan metode spektrofotometri UV-Vis. Kovalen: J Riset Kimia. 2020;6(3):230-38. https://doi.org/10.22487/kovalen.2020.v6.i3.15324
  12. Padmawinata, Kosasih, Soediro, Iwang, Harborne JB. Metode Fitokimia: Penuntun cara modern menganalisis tumbuhan. Bandung: ITB; 1987. http://digilib.ust.ac.id//index.php?p=show_detail&id=6643
  13. Singh K. Pharmacognostical aspects and phytochemical screening of powdered Rosa centifolia petal. J Drug Deliv Ther. 2022;12(2-s):155-63. http://dx.doi.org/10.22270/jddt.v12i2-s.5298
  14. Andika B, Halimatussakdiah, Amna U. Analisis kualitatif senyawa metabolit sekunder ekstrak daun gulma siam (Chlomolaena odorata L.) di kota Langsa, Aceh. Jurnal Kimia Sains dan Terapan. 2020;2(2):1-6. https://doi.org/10.33059/jq.v2i2.2647
  15. Adekanmi AA, Adekanmi SA, Adekanmi OS. Qualitative and quantitative phytochemical constituents of moringa leaf. Int J Eng Inf Syst. 2020;4(5):10-17.
  16. Phuyal N, Jha PK, Raturi PP, Rajbhandary S. Total phenolic, flavonoid contents and antioxidant activities of fruit, seed and bark extracts of Zanthoxylum armatum DC. Sci World J. 2020;8780704:1-7. https://doi.org/10.1155/2020/8780704
  17. Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, et al. Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molecules. 2022;27(4):1326. https://doi.org/10.3390/molecules27041326
  18. Sharma T, Tyagi V, Bansal M. Determination of sun protection factor of vegetable and fruit extracts using UV–Visible spectroscopy: A green approach. Sustain Chem Pharm. 2020;18(3):100347. https://doi.org/10.1016/j.scp.2020.100347
  19. Mileva M, Ilieva Y, Jovtchev G, Gateva S, Zaharieva MM, Georgieva A, et al. Rose flowers—A delicate perfume or a natural healer? Biomolecules. 2021;11(127):1-32. https://doi.org/10.3390/ biom11010127
  20. Latcuba SP, Chiuman L, Nasution AN, Ginting CN. Analysis of wound healing activity from rose petal extract ointment in Wistar rats. In: Proceedings of the IOP Conference Series: Earth and Environmental Science; 2022.1083(012047). p.1-8. IOP Publishing. https://doi.org/10.1088/1755-1315/1083/1/012047
  21. Nimbal SK, Gadad PC, Koti BC. Effect of ethanolic extract of Rosa centifolia against doxorubicin induced nephrotoxicity in albino rats. J Ayurveda Integr Med. 2021;12(4):657–62. https://doi.org/10.1016/j.jaim.2021.07.020
  22. Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, isolation and identification of bioactive compounds from plant extracts. Plants. 2017;6(4):42. https://doi.org/10.3390/plants6040042
  23. Bitwell C, Indra SS, Luke C, Kakoma MK. A review of modern and conventional extraction techniques and their applications for extracting phytochemicals from plants. Scientific African. 2023;19:e01585. https://doi.org/10.1016/j.sciaf.2023.e01585
  24. Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants. 2015;4:3. doi:10.4172/2167- 0412.1000196
  25. Verma A, Srivastava R, Sonar PK, Yadav R. Phytochemical screening, in-silico toxicity profiling and antidiabetic potential of the flowers of Rosa alba L. J Phytopharmacol. 2024;13(2):167–76. https://doi.org/10.31254/phyto.2024.13211
  26. Kandylis P. Phytochemicals and antioxidant properties of edible flowers. Appl Sci. 2022;12(19):9937. https://doi.org/10.3390/app12199937
  27. Panche AN, Diwan AD, Chandra SR. Flavonoids: An overview. J Nutr Sci. 2016;5:e47. https://doi.org/10.1017/jns.2016.41
  28. Patle TK, Shrivas K, Kurrey R, Upadhyay S, Jangde R, Chauhan R. Phytochemical screening and determination of phenolics and flavonoids in Dillenia pentagyna using UV–Vis and FTIR spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc. 2020;5:242. https://doi.org/10.1016/j.saa.2020.118717
  29. Singh K. Determination of phenolics and flavonoids in ethanolic extract of Rosa centifolia using UV-visible Spectroscopy. Nat Vol Essent Oil. 2024;11(02):01-06. https://doi.org/10.53555/nveo.v11i02.5630
  30. Singh K, Patel D. Physiochemical evaluation and determination of chemical constituent in rose petal (Rosa centifolia). J Drug Deliv Ther. 2021;11(3):9–16. https://doi.org/10.22270/jddt.v11i3.4754
  31. Tanjga BB, Loncar B, Acimovic M, Kiprovski B, Å ovljanski O, Tomic A, et al. Volatile profile of garden rose (Rosa hybrida) hydrosol and evaluation of its biological activity in vitro. Horticulturae. 2022;8(10):895. https://doi.org/10.3390/horticulturae8100895
  32. Reviana R, Usman AN, Raya I, Aliyah, Dirpan A, Arsyad A, et al. Analysis of antioxidant activity on cocktail honey products as female pre-conception supplements. Gac Sanit. 2021;35:S202–05. https://doi.org/10.1016/j.gaceta.2021.10.021
  33. Kumar S, Sandhir R, Ojha S. Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Res Notes. 2014;7(1):560. https://doi: 10.1186/1756-0500-7-560
  34. Molyneux P. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. J Sci Technol. 2004;26(2):211-19.
  35. Saroyo H, Arifah IAN. Antioxidant activity using DPPH and FRAP method and their correlation with the levels of phenolic and flavonoid compounds from nemba plants (Azadirachta Indica A. Juss). J Nutraceut Herbal Med. 2020;3(2):10-20.
  36. Mosa FA, Alsaady FZ, Naser HI. Determination of sun protection factor (SPF) number of some aqueous botanical extracts by ultraviolet spectrophotometry. J Sci. 2023;15:1-8.

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