In vitro efficacy of organic amendments and biocontrol agents against Sclerotium rolfsii causing groundnut stem rot disease
DOI:
https://doi.org/10.14719/pst.5238Keywords:
Groundnut, Sclerotium rolfsii , stem rot, organic amendments, biocontrol agentsAbstract
Groundnut is a crucial oil seed crop cultivated worldwide and often referred to as the "King of Oil Seeds." However, its productivity is significantly reduced by various biotic and abiotic stresses. Among these, soil-borne fungal infections, particularly stem rot disease caused by Sclerotium rolfsii Sacc., pose a major threat, potentially leading to yield losses of up to 80 percent. In this study, stem rot-infected samples were collected from five major groundnut-growing districts in Tamil Nadu, India, and nine isolates of S. rolfsii were obtained. Based on pathogenicity tests, the most virulent isolate was identified and characterized at the molecular level. The pathogen produces a resting structure called sclerotia, which survives in soil for many years. Considering the ill effects of chemical methods of management, the present study is focused on non-chemical methods using organic amendments and biocontrol agents against the pathogen. Six amendments, viz., groundnut cake, neem cake, castor cake, cotton cake, sesame cake, and cow manure, were tested against S. rolfsii under in vitro conditions at two concentrations (5 % and 10 %). Among these, sesame cake exhibited the highest inhibition of 48.36 percent and 63.80 percent at 5 percent and 10 percent concentrations, respectively. Through GC-MS analysis, the bioactive compounds, viz., 9,12-Octadecadienoic acid (Z, Z) - (100%) and 9-Octadecenoic acid (E) - (76.13%), responsible for pathogen inhibition were identified. Furthermore, rhizospheric bacterial biocontrol agents were evaluated against S. rolfsii, which revealed that isolate B2 showed maximum inhibition (79.48%). The bacterial isolate B2 was molecularly characterized and confirmed as Bacillus subtilis (PP882830).
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Rathnakumar A, Singh R, Parmar D, Misra J. Groundnut: a crop profile and compendium of notified varieties of India. Directorate of Groundnut Research, India. 2013. https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Rathnakumar+A%2C+Singh+R%2C+Parmar+D%2C+Misra+J.+Groundnut%3A+a+crop+profile+and+compendium+of+notified+varieties+of+India.+Directorate+of+Groundnut+Research%2C+India.+2013.&btnG=
El Naim AM, Eldoma M, Abdalla AE. Effect of weeding frequencies and plant density on vegetative growth characteristic of groundnut (Arachis hypogaea L.) in North Kordofan of Sudan. International Journal of Applied Biology and Pharmaceutical Technology. 2010;1(3):1188-93. https://www.researchgate.net/profile/Ahmed-El-Naim/publication/233950650_EFFECT_OF_WEEDING_FREQUENCIES_AND_PLANT_DENSITY_ON_THEVEGETATIVE_GROWTH_CHARACTERISTIC_IN_GROUNDNUT_Arachis_hypogaea_LIN_NORTH_KORDOFAN_OF_SUDAN/links/02bfe50d3ffb9cd5f6000000/EFFECT-OF-WEEDING-FREQUENCIES-AND-PLANT-DENSITY-ON-THE-VEGETATIVE-GROWTH-CHARACTERISTIC-IN-GROUNDNUT-Arachis-hypogaea-L-IN-NORTH-KORDOFAN-OF-SUDAN.pdf
https://www.fao.org/faostat/en/
Vineela D, Beura S, Dhal A, Swain S. Integrated management of soil borne diseases of groundnut in coastal ecosystem of Odisha. Journal of Mycopathological Research. 2018;56(3):189-93. https://www.imskolkata.org/pdf/oct_18/D_R_S.pdf
Jadon K, Thirumalaisamy P, Kumar V, Koradia V, Padavi R. Management of soil borne diseases of groundnut through seed dressing fungicides. Crop Protection. 2015;78:198-203. https://doi.org/10.1016/j.cropro.2015.08.021
Ghewande M, Desai S, Basu MS. Diagnosis and management of major disease of groundnut. Proceedings of a Meet September. 2002;13-14. https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Ghewande+M.+Desai%2C+S.+and+Basu%2C+MS+2002&btnG=
Manasa P, Senapati A, Kumar S, Dwibedi SK. Identifying the susceptibility stage of groundnut plant to stem rot disease. 2022 https://www.researchgate.net/profile/Sanat-Dwibedi/publication/364575074_Identifying_the_susceptibility_stage_of_groundnut_plant_to_stem_rot_disease/links/635257586e0d367d91aff1f7/Identifying-the-susceptibility-stage-of-groundnut-plant-to-stem-rot-disease.pdf
Hawaladar S, Nandan M, Vinaykumar H, Hadimani RH, Hiremath S, Venkataravanappa V, et al. Morphological and molecular characterization of Sclerotium rolfsii associated with stem rot disease of groundnut (Arachis hypogaea L.). Indian Phytopathology. 2022;1-12. https://doi.org/10.1007/s42360-021-00419-y
Sunkad G. Tebuconazole: a new triazole fungicide molecule for the management of stem rot of groundnut caused by Sclerotium rolfsii. The Bioscan. 2012;7(4):601-03. https://thebioscan.com/index.php/pub/article/download/1099/1052
Rangaswami G, Mahadevan A. Diseases of crop plants in India. PHI Learning Pvt. Ltd.; 1998. https://books.google.com/books?hl=en&lr=&id=4yb-VnjZTycC&oi=fnd&pg=PP13&dq=Rangaswami+G,+Mahadevan+A.+Diseases+of+crop+plants+in+India:+PHI+Learning+Pvt.+Ltd.%3B+1998.&ots=TsPfezwAbq&sig=mp56duObin7yJJlespfBLrxbiwU
Paul NC, Kim W-K, Woo S-K, Park M-S, Yu S-H. Fungal endophytes in roots of Aralia species and their antifungal activity. The Plant Pathology Journal. 2007;23(4):287-94. https://doi.org/10.5423/PPJ.2007.23.4.287
Tutte J. Plant pathological methods: Fungi and Bacteria. Minneapolis, Minn. Burgess Publishing Com; 1969.
Punja ZK. The biology, ecology and control of Sclerotium rolfsii. Annual Review of Phytopathology. 1985;23(1):97-127. https://doi.org/10.1146/annurev.py.23.090185.000525
Jebaraj MD, Aiyanathan KEA, Nakkeeran S. Virulence and genetic diversity of Sclerotium rolfsii Sacc., infecting groundnut using nuclear (RAPD and ISSR) markers. Journal of Environmental Biology. 2017;38(1):147. https://doi.org/10.22438/jeb/38/1/ms-274
Zhang Y-p, Uyemoto J, Kirkpatrick B. A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virological Methods. 1998;71(1):45-50. https://doi.org/10.1016/S0166-0934(97)00190-0
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 2013;30(12):2725-29. https://doi.org/10.1093/molbev/mst197
Kuldhar D, Suryawanshi A. Integrated management of stem rot and pod rot (Sclerotium rolfsii) of groundnut (Arachis hypogaea L.). Agric Update. 2017;12:238-46. https://doi.org/10.15740/HAS/AU/12.TECHSEAR(1)2017/238-246
Vincent J. The esters of 4?hydroxybenzoic acid and related compounds. Part I. Methods for the study of their fungistatic properties. Journal of the Society of Chemical Industry. 1947;66(5):149-55. https://doi.org/10.1002/jctb.5000660504
Almutairi FM, Khan A, Ajmal MR, Khan RH, Khan MF, Lal H, et al. Phytochemical analysis and binding interaction of cotton seed cake derived compounds with target protein of Meloidogyne incognita for nematicidal evaluation. Life. 2022;12(12):2109. https://doi.org/10.3390/life12122109
Dennis C, Webster J. Antagonistic properties of species-groups of Trichoderma: I. Production of non-volatile antibiotics. Transactions of the British Mycological Society. 1971;57(1):25-IN3. https://doi.org/10.1016/S0007-1536(71)80077-3
Simmons J. A culture method for differentiating organisms of typhoid colour aerogenes group and for isolation of certain fungi. J Infect Dis. 1976;4:39-209. https://doi.org/10.1093/infdis/39.3.209
O'meara R. A Simple delicate and rapid method of detecting the formation of acetylmethylcarbinol by bacteria fermenting carbohydrate. 1931. https://doi.org/10.1002/path.1700340402
Seeley HW, VanDemark PJ. Microbes in action: A laboratory manual of microbiology. (No Title). 1970. https://www.cabidigitallibrary.org/doi/full/10.5555/19620403696
Blazevic DJ, Ederer G. Principles of biochemical tests in diagnostic microbiology. 1975. https://www.sidalc.net/search/Record/KOHA-OAI-UAAAN:14462/Description
Makwana GE, Gadhavi H, Sinha M. Comparision of tube coagulase test with mannitol fermentation test for diagnosis of Staphylococcus aureus. 2012. https://pesquisa.bvsalud.org/portal/resource/pt/sea-152213
Ahmed A, Kazmi S. Siderophore production and its role as therapeutic agent. Microbiological and Immunological Communications. 2022;1(1):21-33. https://doi.org/10.55627/mic.001.01.0181
Thind T. Diseases of field crops and their management. Daya Books; 2005. https://books.google.com/books?hl=en&lr=&id=6ELqjS-qg9IC&oi=fnd&pg=PA1&dq=Thind+T.+Diseases+of+field+crops+and+their+management:+Daya+Books%3B+2005&ots=Y6CtQ9uUvy&sig=47R5CZctQRCfjkxvW1L-JngPMqI
El-Nagar AAA, Sabry A, Yassin MA. Virulence and host range of Sclerotium rolfsii and S. cepivorum. Journal of Pure and Applied Microbiology. 2013;7(3):1693-705. https://www.cabidigitallibrary.org/doi/full/10.5555/20133417267
Punja Z, Grogan R. Hyphal interactions and antagonism among field isolates and single-basidiospore strains of Athelia (Sclerotium) rolfsii. Phytopathology. 1983;73(9):1279-84. https://doi.org/10.1094/Phyto-73-1279
Punja Z, Huang J-S, Jenkins S. Relationship of mycelial growth and production of oxalic acid and cell wall degrading enzymes to virulence in Sclerotium rolfsii. Canadian Journal of plant pathology. 1985;7(2):109-17. https://doi.org/10.1080/07060668509501485
Rasu T, Sevugapperumal N, Thiruvengadam R, Ramasamy S. Morphological and genomic variability among Sclerotium rolfsii populations. The Bioscan. 2013;8(4):1425-30. https://thebioscan.com/index.php/pub/article/view/452
Akram M, Saabale P, Kumar A, Chattopadhyay C. Morphological, cultural and genetic variability among Indian populations of Sclerotium rolfsii. Journal of Food Legumes. 2015;28(4):330-34. https://www.indianjournals.com/ijor.aspx?target=ijor:jfl&volume=28&issue=4&article=011
Paul NC, Hwang E-J, Nam S-S, Lee H-U, Lee J-S, Yu G-D, et al. Phylogenetic placement and morphological characterization of Sclerotium rolfsii (Teleomorph: Athelia rolfsii) associated with blight disease of Ipomoea batatas in Korea. Mycobiology. 2017;45(3):129-38. https://doi.org/10.5941/MYCO.2017.45.3.129
Le C, Mendes R, Kruijt M, Raaijmakers J. Genetic and phenotypic diversity of Sclerotium rolfsii in groundnut fields in central Vietnam. Plant Disease. 2012;96(3):389-97. https://doi.org/10.1094/PDIS-06-11-0468
Mahadevakumar S, Janardhana G. Morphological and molecular characterization of Sclerotium rolfsii associated with leaf blight disease of Psychotria nervosa (wild coffee). Journal of Plant Pathology. 2016:351-54. https://www.jstor.org/stable/44280457
Rahman MT, Bhuiyan MKA, Akanda MAM, Khan MAA, Karim MA, Hossain MM, et al. Integrated approaches for managing collar rot disease and increasing soybean yield. Egyptian Journal of Agricultural Research. 2024;102(1):90-102. https://ejar.journals.ekb.eg/article_316957.html
Matisic M, Dugan I, Bogunovic I. Challenges in sustainable agriculture—The role of organic amendments. Agriculture. 2024 Apr 22;14(4):643. https://doi.org/10.3390/agriculture14040643 https://www.mdpi.com/2077-0472/14/4/643
Ali A, Javaid A, Shoaib A. GC-MS analysis and antifungal activity of methanolic root extract of Chenopodium album against Sclerotium rolfsii. Planta Daninha. 2017;35:e017164713. https://doi.org/10.1590/s0100-83582017350100046
Ayyandurai M, Akila R, Manonmani K, Harish S, Mini M, Vellaikumar S. Deciphering the mechanism of Trichoderma spp. consortia possessing volatile organic compounds and antifungal metabolites in the suppression of Sclerotium rolfsii in groundnut. Physiological and Molecular Plant Pathology. 2023;125:102005. https://doi.org/10.1016/j.pmpp.2023.102005
Handelsman J, Stabb EV. Biocontrol of soilborne plant pathogens. The Plant Cell. 1996;8(10):1855. https://doi.org/10.2307/3870235
Collins DP, Jacobsen BJ, Maxwell B. Spatial and temporal population dynamics of a phyllosphere colonizing Bacillus subtilis biological control agent of sugar beet Cercospora leaf spot. Biological Control. 2003;26(3):224-32. https://doi.org/10.1016/S1049-9644(02)00146-9
Gardener BBM. Ecology of Bacillus and Paenibacillus spp. in agricultural systems. Phytopathology. 2004;94(11):1252-58. https://doi.org/10.1094/PHYTO.2004.94.11.1252
Jayaraj J, Radhakrishnan N, Kannan R, Sakthivel K, Suganya D, Venkatesan S, et al. Development of new formulations of Bacillus subtilis for management of tomato damping-off caused by Pythium aphanidermatum. Biocontrol Science and Technology. 2005;15(1):55-65. https://doi.org/10.1080/09583150400015920
Earl AM, Losick R, Kolter R. Ecology and genomics of Bacillus subtilis. Trends in Microbiology. 2008;16(6):269-75. https://doi.org/10.1016/j.tim.2008.03.004
Ryu CM, Farag MA, Hu CH, Reddy MS, Kloepper JW, Paré PW. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiology. 2004 Mar 1;134(3):1017-26. https://doi.org/10.1104/pp.103.026583 https://academic.oup.com/plphys/article-abstract/134/3/1017/6112257
Lee KJ, Kamala-Kannan S, Sub HS, Seong CK, Lee GW. Biological control of Phytophthora blight in red pepper (Capsicum annuum L.) using Bacillus subtilis. World Journal of Microbiology and Biotechnology. 2008 Jul;24:1139-45. https://doi.org/10.1007/s11274-007-9585-2 https://link.springer.com/article/10.1007/s11274-007-9585-2
Yao YQ, Lan F, Qiao YM, Wei JG, Huang RS, Li LB. Endophytic fungi harbored in the root of Sophora tonkinensis Gapnep: Diversity and biocontrol potential against phytopathogens. MicrobiologyOpen. 2017 Jun;6(3):e00437. https://doi.org/10.1002/mbo3.437 https://onlinelibrary.wiley.com/doi/abs/10.1002/mbo3.437
Cazorla FM, Romero D, Pérez?García A, Lugtenberg BJ, Vicente AD, Bloemberg G. Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity. Journal of Applied Microbiology. 2007 Nov 1;103(5):1950-59. https://doi.org/10.1111/j.1365-2672.2007.03433.x https://academic.oup.com/jambio/article-abstract/103/5/1950/6719380
Il Kim P, Chung KC. Production of an antifungal protein for control of Colletotrichum lagenarium by Bacillus amyloliquefaciens MET0908. FEMS Microbiology Letters. 2004 May 1;234(1):177-83. https://doi.org/10.1016/j.femsle.2004.03.032 https://academic.oup.com/femsle/article-abstract/234/1/177/577967
Rasool U, Ahmad A, Badroo G, Mudasir M, Fayaz S, Mustafa R. Isolation and identification of Bacillus cereus from fish and their handlers from Jammu, India. International Journal Current Microbiology Applied Science. 2017;6:441-47. https://doi.org/10.20546/ijcmas.2017.608.058
Kalam S, Basu A, Podile AR. Functional and molecular characterization of plant growth promoting Bacillus isolates from tomato rhizosphere. Heliyon. 2020;6(8). https://doi.org/10.1016/j.heliyon.2020.e04734
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