In vitro assessment of entomopathogenic potential of indigenous Clonostachys rosea CR 02 against the whitefly Bemisia tabaci in Potato
DOI:
https://doi.org/10.14719/pst.7278Keywords:
biocontrol, Bemisia tabaci, Clonostachys rosea CR 02, entomopathogenic fungi, SEMAbstract
Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) are economically important polyphagous pest species causing severe yield losses in Potato. To develop a biopesticide in an environmentally safe manner, the virulence and pathogenecity of the indigenous Clonostachys rosea CR 02 (Ascomycota, Hypocreales: Bionectriaceae) was assessed against whitefly under laboratory condition. The mortality was assessed at varying conidial concentrations and exposure times. Both factors, along with their interaction had significantly influenced, where the higher concentrations and longer exposure times increasing the mortality rate. Maximum mortality of B. tabaci nymphs of 75.83% at 1.0 × 108 conidia/mL on day 10, with median LC50 values of 3.24 × 106 and LT50 values of 1.0 × 108 was 6.82 day. For adult B. tabaci, mortality increased exponentially, reaching 52.5% by day 10 at a concentration of 1.0 × 108 conidia/mL. However, C. rosea CR 02 showed lower virulence against adults compared to nymphs. Scanning electron microscopy (SEM) images of infected nymph B. tabaci revealed conidial adherence, hyphal growth, hyphal penetration, conidiophore formation and completely colonized the host’s surface (24-96 hours post-inoculation). For adult B. tabaci, mycelial colonization observed 7 days after treatment. This study highlights the entomopathogenic potential of indigenous C. rosea CR 02 against B. tabaci and suggests their use for the environmentally sustainable pest management.
Downloads
References
Mishra P, Emam W, Tashkandy Y, Panchabhai S, Srivastava AB. State of art on potato production in South Asian countries and their yield sustainability. Potato Research. 2024;1-25. https://doi.org/10.1007/s11540-024-09759-4
Sahu PK, Das M, Sarkar B, VS A, Dey S, Narasimhaiah L, Mishra P, Tiwari R, Raghav YS. Potato production in India: a critical appraisal on sustainability, forecasting, price and export behaviour. Potato Research. 2024;1-37. https://doi.org/10.1007/s11540-023-09682-0
Shah MA. Efficacy of new-chemistry insecticides against whitefly and aphids in potato. Potato Journal. 2022;49(1). https://doi.org/10.55446/IJE.2022.162
Bairwa A, Naga KC, Sharma S, Kumar A. Management of major insects and nematodes of potato. Indian Farming. 2024;74(5): 59-63.
Singh H, Kaur T. Pathogenicity of entomopathogenic fungi against the aphid and the whitefly species on crops grown under greenhouse conditions in India. Egyptian Journal of Biological Pest Control. 2020;30(1):84. https:// doi. org/ 10. 1186/ s41938- 020- 00287-0
Bass C, Nauen R. The molecular mechanisms of insecticide resistance in aphid crop pests. Insect Biochemistry and Molecular Biology. 2023;156:103937. https://doi.org/10.1016/j.ibmb.2023.103937
Gomes HDO, Menezes JMC, da Costa JGM, Coutinho HDM, Teixeira RNP, do Nascimento RF. A socio-environmental perspective on pesticide use and food production. Ecotoxicology and Environmental Safety. 2020;197:110627. https://doi.org/10.1016/j.ecoenv.2020.110627
Ullah S, Raza ABM, Alkafafy M, Sayed S, Hamid MI, Majeed MZ, et al. Isolation, identification and virulence of indigenous entomopathogenic fungal strains against the peach-potato aphid, Myzus persicae Sulzer (Hemiptera: Aphididae), and the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control. 2022; 32(1):1-11. https://doi.org/10.1186/s41938-021-00500-8
Krishnaveni M, Jeyarajan NS, Nakkeeran S, Sivakumar U, Mohankumar S, Premalatha K. Entomopathogenic potential of indigenous Simplicillium subtropicum (Hypocreales: Cordycipitaceae) isolates from Tamil Nadu, India, against the cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae). Egyptian Journal of Biological Pest Control. 2024; 34(1):55. https://doi.org/10.1186/s41938-024-00820-5
Leger RJS, Wang C, Fang W. New perspectives on insect pathogens. Fungal Biology Reviews. 2011;25(2):84-8. https://doi.org/10.1016/j.fbr.2011.04.005
Dara SK. Non-entomopathogenic roles of entomopathogenic fungi in promoting plant health and growth. Insects. 2019;10(9):277. https://doi.org/10.3390/insects10090277
Sun ZB, Li SD, Ren Q, Xu JL, Lu X, Sun MH. Biology and applications of Clonostachys rosea. Journal of Applied Microbiology. 2020;129(3):486-95. https://doi.org/10.1111/jam.14625
Muvea AM, Meyhofer R, Subramanian S, Poehling HM, Ekesi S, Maniania NK. Colonization of onions by endophytic fungi and their impacts on the biology of Thrips tabaci. PloS one 2014;9(9):e108242. https://doi.org/10.1371/journal.pone.0108242
Hamiduzzaman MM, Sinia A, Guzman-Novoa E, Goodwin PH. Entomopathogenic fungi as potential biocontrol agents of the ecto-parasitic mite, Varroa destructor, and their effect on the immune response of honeybees (Apis mellifera L.). Journal of Invertebrate Pathology. 2012;111(3):237-43. https://doi.org/ 10.1016/j.jip.2012.09.001
Tamta AK, Pandey R, Sharma JR, Rai R, Barman M, Deeksha MG, et al. First record of Clonostachys rosea (Ascomycota: Hypocreales) entomopathogenic fungus in the Mango Hopper Amritodus atkinsoni (Hemiptera: Cicadellidae). Pathogens. 2022;11(12):1447. https://doi.org/10.3390/pathogens11121447
Al-Nabhani SS, Kazerooni EA, Al-Raqmi S, Al-Hashmi M, Hussain S, Velazhahan R, et al. Isolation of Clonostachys rosea and characterizing its entomopathogenic activity against Dubas bug (Ommatissus lybicus) nymphs and adults. Agriculture. 2024;14(10):1770. https://doi.org/10.3390/agriculture14101770
Maistrou S, Natsopoulou ME, Jensen AB, Meyling NV. Virulence traits within a community of the fungal entomopathogen Beauveria: Associations with abundance and distribution. Fungal Ecology. 2020;48:100992. https://doi.org/10.1016/j.funeco.2020.100992
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology. 1994; 3: 294-99.
Mascarin GM, Kobori NN, Quintela ED, Delalibera I. The virulence of entomopathogenic fungi against Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) and their conidial production using solid substrate fermentation. Biocontrol.2013; 66(3):209-18. https://doi.org/10.1016/j.biocontrol.2013.05.001
Sani I, Jamian S, Saad N, Abdullah S, Mohd Hata E, Jalinas J, et al. Identification and virulence of entomopathogenic fungi, Isaria javanica and Purpureocillium lilacinum isolated from the whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Malaysia. Egyptian Journal of Biological Pest Control. 2023;33(1):14. https://doi.org/10.1186/s41938-023-00657-4
Tian J, Diao H, Liang L, Hao C, Arthurs S, Ma R. Pathogenicity of Isaria fumosorosea to Bemisia tabaci, with some observations on the fungal infection process and host immune response. Journal of Invertebrate Pathology. 2015;130:147-53. https://doi.org/10.1016/j.jip.2015.08.003
Gomez KA, Gomez AA. Statistical procedures for agricultural research. Wiley India Private Limited. India. 2010;165-68.
Finney DJ. Statistical logic in the monitoring of reactions to therapeutic drugs. Methods of Information in Medicine. 1971;10(04):237–45.
Kaur R, Choudhary D, Bali S, Bandral SS, Singh V, Ahmad MA, et al. Pesticides: An alarming detrimental to health and environment. Science of The Total Environment. 2024;915:170113. https://doi.org/10.1016/j.scitotenv.2024.170113
Dash CK, Bamisile BS, Keppanan R, Qasim M, Lin Y, Islam SU, et al. Endophytic entomopathogenic fungi enhance the growth of Phaseolus vulgaris L. (Fabaceae) and negatively affect the development and reproduction of Tetranychus urticae Koch (Acari: Tetranychidae). Microbial Pathogenesis. 2018;125:385-92. https://doi.org/10.1016/j.micpath.2018.09.044
Toledo AV, Virla E, Humber RA, Paradell SL, Lastra CL. First record of Clonostachys rosea (Ascomycota: Hypocreales) as an entomopathogenic fungus of Oncometopia tucumana and Sonesimia grossa (Hemiptera: Cicadellidae) in Argentina. Journal of Invertebrate Pathology. 2006;92(1):7-10. https://doi.org/10.3390/pathogens11121447

Downloads
Published
Versions
- 23-02-2025 (2)
- 15-02-2025 (1)
How to Cite
Issue
Section
License
Copyright (c) 2025 J Shajith Basha, V K Parthiban, M Karthikeyan , I Johnson, T Elaiyabharathi , Priyank Hanuman Mahtre, R Anandham , M Jayakanthan

This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).